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. 2023 Mar 14;10(3):ofad140.
doi: 10.1093/ofid/ofad140. eCollection 2023 Mar.

Utility of a Viral Vesicular Panel Multiplex Polymerase Chain Reaction Assay for the Diagnosis of Monkeypox, Herpes Simplex, and Varicella Zoster Viruses

Eli Wilber  1 , Paulina A Rebolledo  1   2   3 , Vyjayanti Kasinathan  4 , Stephanie Merritt  5 , Boghuma K Titanji  1 , Bruce Aldred  1 , Sheetal Kandiah  1 , Susan M Ray  1   2   3 , Anandi N Sheth  1   3 , Jonathan A Colasanti  1   2   3 , Yun F Wang  4   5
Affiliations

Utility of a Viral Vesicular Panel Multiplex Polymerase Chain Reaction Assay for the Diagnosis of Monkeypox, Herpes Simplex, and Varicella Zoster Viruses

Eli Wilber et al. Open Forum Infect Dis. .

Abstract

Mpox (monkeypox) represents a diagnostic challenge due to varied clinical presentations and multiple mimics. A commercially available multiplex polymerase chain reaction panel accurately detects mpox virus as well as common mimics (herpes simplex virus, varicella zoster virus) in clinical specimens and could be used in routine clinical, surveillance, and outbreak settings.

Keywords: mpox; mpox diagnosis; multiplex PCR assay; vesicular panel.

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Conflict of interest statement

Potential conflicts of interest. B. K. T. received consulting fees from the nonprofit CRITICA, unrelated to this work. All other authors report no potential conflicts.

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