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. 2023 Mar 16;67(1):33-40.
doi: 10.2478/jvetres-2023-0010. eCollection 2023 Mar.

Serum and Milk Levels of Antibodies to Bovine Viral Diarrhoea Virus, Bovine Herpesvirus-1 and -4, and Circulation of Different Bovine Herpesvirus-4 Genotypes in Dairy Cattle with Clinical Mastitis

Veysel S Ataseven  1 , Pınar Ambarcıoğlu  2 , Fırat Doğan  1
Affiliations

Serum and Milk Levels of Antibodies to Bovine Viral Diarrhoea Virus, Bovine Herpesvirus-1 and -4, and Circulation of Different Bovine Herpesvirus-4 Genotypes in Dairy Cattle with Clinical Mastitis

Veysel S Ataseven et al. J Vet Res. .

Abstract

Introduction: Bovine viral diarrhoea virus (BVDV) and bovine herpesvirus (BoHV)-1 and -4 are important causes of respiratory diseases and reproductive disorders of dairy cattle worldwide.

Material and methods: Investigation of BVDV and BoHV-1 and -4 antibody levels in the serum and milk of dairy cattle in a group with clinical mastitis and a healthy group was undertaken using an indirect ELISA, and identification of the BoHV-4 genotypes in clinical mastitis cases was attempted by PCR and sequencing.

Results: Antibodies specific to BVDV, BoHV-1 and BoHV-4 were detected in the serum and milk of all dairy cattle with clinical mastitis. The cut-off values for BVDV and BoHV-1 in the sera and milk were extremely high in both healthy and mastitic animals. However, BoHV-4 antibodies were detected only in the clinically mastitic cattle, and BoHV-4 levels were higher in milk than in sera among these animals. Genotypes I and II of BoHV-4 were detected in the milk samples of four seropositive cows with clinical mastitis from the same herd.

Conclusion: The results of this investigation demonstrate that clinical mastitis cases in the same herd may have aetiology in different BoHV-4 genotypes.

Keywords: antibody; bovine herpesvirus-1; bovine herpesvirus-4; bovine viral diarrhoea virus; mastitis.

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Conflict of interest statement

Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
Changes in bovine viral diarrhoea virus (A) and bovine herpesvirus-1 (B) levels between serum and milk samples collected from cattle with clinical mastitis and healthy cattle
Fig. 2
Fig. 2
Bland–Altman plot with 95% limit of agreement of serum and milk samples analysed for bovine viral diarrhoea virus expressed as sample/ positive ratio in cattle with clinical mastitis (A) and healthy cattle (B)
Fig. 3
Fig. 3
Bland–Altman plot with 95% limit of agreement of serum and milk samples analysed for bovine herpesvirus-1 expressed as percent positivity in cattle with clinical mastitis (A) and healthy cattle (B)
Fig. 4
Fig. 4
Bland–Altman plot with 95% limit of agreement of serum and milk samples analysed for bovine herpesvirus-4 expressed as percent positivity in cattle with clinical mastitis
Fig. 5
Fig. 5
Phylogenetic tree for the partial gB gene of bovine herpesvirus-4 strains after sequence alignment compared with other sequences deposited in the GenBank database. Bar – number of base substitutions per site
See this image and copyright information in PMC

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