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. 2023 Jun;58(6):813-822.
doi: 10.1111/rda.14354. Epub 2023 Apr 12.

Molecular and physiochemical evaluation of buck semen cryopreserved with antioxidants

Nasser Ghanem  1   2 , Norhan Hamdy Nasr  3 , Nehal Ali Abu Elnaga  3 , Fatma Abou-Hashim  1 , Shaimaa Kamel  4 , Mohamad Warda  4 , Sherif M Dessouki  1 , Mervat Ahmed AbdRabou  5 , Gamal M K Mehaisen  1
Affiliations

Molecular and physiochemical evaluation of buck semen cryopreserved with antioxidants

Nasser Ghanem et al. Reprod Domest Anim. 2023 Jun.

Abstract

The current study evaluated the physiochemical quality and gene expression profile of post-thawed buck semen after supplementation with antioxidants [melatonin (M), L-carnitine (LC), cysteine (Cys), LC + M, M + Cys, LC + Cys, LC + Cys + M] in comparison with the non-treated control group. Physical and biochemical characteristics of semen were evaluated following freezing and thawing. Transcript abundance of six selected candidate genes was profile using quantitative real-time PCR. The data demonstrated significant enhancement of post-freezing total motility, progressive motility, percentage of live sperm, CASA parameters, plasma membrane and acrosome integrity in all groups supplemented with Cys, LC, M + Cys and LC + Cys compared with the control group. The biochemical analysis of semen indicated that semen groups supplemented with LC and LC + Cys recorded increased levels of GPX and SOD that were coupled with up-regulation of antioxidant genes (SOD1, GPX1 and NRF2) and mitochondrial transcripts (CPT2 and ATP5F1A). Moreover, H2O2 level and DNA fragmentation percentage were reduced compared with other groups. In conclusion, supplementation of Cys alone or in combination with LC positively improved the post-thaw physiochemical properties of rabbit semen through activation of bioenergetics-related mitochondrial genes and cellular antioxidant defence mechanism.

Keywords: antioxidants; biochemical; bucks; physical; transcript abundance.

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References

REFERENCES

    1. Abad, C., Amengual, M. J., Gosálvez, J., Coward, K., Hannaoui, N., Benet, J., García-Peiró, A., & Prats, J. (2013). Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA. Andrologia, 45, 211-216.
    1. Aliabadi, E., Mehranjani, S. M., Borzoei, Z., Talaei-Khozani, T., Mirkhani, H., & Tabesh, H. (2012). Effects of L-carnitine and L-acetyl-carnitine on testicular sperm motility and chromatin quality. Iranian Journal of Reproductive Medicine, 10(2), 77-82.
    1. Arafa, M., Agarwal, A., Majzoub, A., Selvam, M. K. P., Baskaran, S., Henkel, R., & Elbardisi, H. (2020). Efficacy of antioxidant supplementation on conventional and advanced sperm function tests in patients with idiopathic male infertility. Antioxidants, 9(3), 219.
    1. Boerke, A., Dieleman, S. J., & Gadella, B. M. (2007). A possible role for spermatozoa RNA in early embryo development. Theriogenology, 68, 147-155.
    1. Chen, X., Zhu, H., Hu, C., Hao, H., Zhang, J., Li, K., Zhao, X., Qin, T., Zhao, K., & Wang, D. (2014). Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC-MS/MS. Reproduction, 147, 321-330.

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