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. 2023 Aug 10;142(6):509-518.
doi: 10.1182/blood.2022018071.

Human CD34+ hematopoietic stem cell hierarchy: how far are we with its delineation at the most primitive level?

Fernando Anjos-Afonso  1   2 , Dominique Bonnet  2
Affiliations

Human CD34+ hematopoietic stem cell hierarchy: how far are we with its delineation at the most primitive level?

Fernando Anjos-Afonso et al. Blood. .

Abstract

The ability to isolate and characterize different hematopoietic stem cell (HSC) or progenitor cell populations opens avenues to understand how hematopoiesis is regulated during development, homeostasis, and regeneration as well as in age-related conditions such as clonal hematopoiesis and leukemogenesis. Significant progress has been made in the past few decades in determining the composition of the cell types that exist in this system, but the most significant advances have come from mouse studies. However, recent breakthroughs have made significant strides that have enhanced the resolution of the human primitive hematopoietic compartment. Therefore, we aim to review this subject not only from a historical perspective but also to discuss the progress made in the characterization of the human postnatal CD34+ HSC-enriched populations. This approach will enable us to shed light on the potential future translational applicability of human HSCs.

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Conflict of interest statement

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Figures

None
Graphical abstract
Figure 1.
Figure 1.
Hematopoietic stem cell markers and their potential signaling interactions governing their biology. A schematic diagram of potential signaling pathways downstream of key markers expressed on CD34+(CD38/CD45RA)–EPCR+ HSCs (which are also CD49f+ and CD90+/−) upon interacting with their potential ligands in the niche. This highly HSC-enriched population highly expresses CD33, CD93, CD117, CD133, and CD143. We hypothesize that these cells may also express GPRC5C and CD370 as well as PAR1 and ATR1 (to convey the EPCR/APC/PAR1 and CD143/Ang-/ATR1 signaling, respectively).
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Comment in

References

    1. Larochelle A, Savona M, Wiggins M, et al. Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers. Blood. 2011;117(5):1550–1554. - PMC - PubMed
    1. Doulatov S, Notta F, Laurenti E, Dick JE. Hematopoiesis: a human perspective. Cell Stem Cell. 2012;10(2):120–136. - PubMed
    1. Laurenti E, Gottgens B. From haematopoietic stem cells to complex differentiation landscapes. Nature. 2018;553(7689):418–426. - PMC - PubMed
    1. Bhatia M, Bonnet D, Murdoch B, Gan OI, Dick JE. A newly discovered class of human hematopoietic cells with SCID-repopulating activity. Nat Med. 1998;4(9):1038–1045. - PubMed
    1. Anjos-Afonso F, Currie E, Palmer HG, Foster KE, Taussig DC, Bonnet D. CD34(-) cells at the apex of the human hematopoietic stem cell hierarchy have distinctive cellular and molecular signatures. Cell Stem Cell. 2013;13(2):161–174. - PubMed

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