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. 2023 Aug;123(4):1321-1329.
doi: 10.1007/s13760-023-02205-1. Epub 2023 Apr 5.

miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury

Meng Wang  1 , Xin Yu  1 , Bin Li  2 , Chensong Gao  1 , Yan Chen  1 , Xiaoyang Zhang  3 , Wenling Li  1 , Lijun Yang  4 , Zhenzeng Fan  5
Affiliations

miR-211-5p targeting MMP9 regulates the expressions of AQP4 in traumatic brain injury

Meng Wang et al. Acta Neurol Belg. 2023 Aug.

Abstract

Objective: The abnormal expression of matrix metalloproteinase 9 (MMP9) and Aquaporin 4 (AQP4) closely associates with the traumatic brain injury (TBI) development.

Methods: Here, we investigated the relationship between miR-211-5p and MMP9/AQP4 axis in TBI patients and astrocyte cells. Demographics, clinical features, and cerebrospinal fluid (CSF) samples were collected from traumatic brain injury (TBI) patients (n = 96) and controls (n = 30) for pathological and gene expression analyses. Luciferase activity assay and gene expression analyses were performed to dissect the regulatory mechanism of miR-211-5p on MMP9/AQP4 in human astrocyte cells.

Results: miR-211-5p mRNA was significantly decreased in the CSF of TBI patients, which positively correlated with the expression of both MMP9 and AQP4. miR-211-5p could target MMP9 directly in SVG P12 cells. Overexpression of miR-211-5p decreased the expression of MMP9, on the contrary, knockdown miR-211-5p through inhibitors increased the expression of both MMP9 and AQP4.

Conclusion: miR-211-5p inhibits the MMP9/AQP4 axis in human astrocyte cells, which represents a promising approach for the TBI treatment.

Keywords: AQP4; Astrocyte cell; MMP9; Traumatic brain injury; miR-211-5p.

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References

    1. Khellaf A, Khan DZ, Helmy A (2019) Recent advances in traumatic brain injury. J Neurol 266:2878–2889 - DOI - PubMed - PMC
    1. Hyder AA, Wunderlich CA, Puvanachandra P, Gururaj G, Kobusingye OC (2007) The impact of traumatic brain injuries: a global perspective. NeuroRehabilitation 22:341–353 - DOI - PubMed
    1. Langlois JA, Rutland-Brown W, Wald MM (2006) The epidemiology and impact of traumatic brain injury: a brief overview. J Head Trauma Rehabil 21:375–378 - DOI - PubMed
    1. Reith FCM, Lingsma HF, Gabbe BJ, Lecky FE, Roberts I, Maas AIR (2017) Differential effects of the Glasgow coma scale score and its components: an analysis of 54,069 patients with traumatic brain injury. Injury 48:1932–1943 - DOI - PubMed
    1. Zafonte RD, Hammond FM, Mann NR, Wood DL, Black KL, Millis SR (1996) Relationship between Glasgow coma scale and functional outcome. Am J Phys Med Rehabil 75:364–369 - DOI - PubMed

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