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. 2023 Jul-Aug;39(4):e3344.
doi: 10.1002/btpr.3344. Epub 2023 Apr 6.

Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid-fortified Hestrin-Schramm medium

Nhu-Ngoc Nguyen  1 , Thi Tuong Vi Tran  1 , Quoc-Duy Nguyen  1 , Tran-Phong Nguyen  1 , Tuyet-Ngan Lien  1
Affiliations

Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid-fortified Hestrin-Schramm medium

Nhu-Ngoc Nguyen et al. Biotechnol Prog. 2023 Jul-Aug.

Abstract

Bacterial cellulose (BC) is a biopolymer with applications in numerous industries such as food and pharmaceutical sectors. In this study, various hydrocolloids including modified starches (oxidized starch-1404 and hydroxypropyl starch-1440), locust bean gum, xanthan gum (XG), guar gum, and carboxymethyl cellulose were added to the Hestrin-Schramm medium to improve the production performance and microstructure of BC by Gluconacetobacter entanii isolated from coconut water. After 14-day fermentation, medium supplemented with 0.1% carboxymethyl cellulose and 0.1% XG resulted in the highest BC yield with dry BC content of 9.82 and 6.06 g/L, respectively. In addition, scanning electron microscopy showed that all modified films have the characteristic three-dimensional network of cellulose nanofibers with dense structure and low porosity as well as larger fiber size compared to control. X-ray diffraction indicated that BC fortified with carboxymethyl cellulose exhibited lower crystallinity while Fourier infrared spectroscopy showed characteristic peaks of both control and modified BC films.

Keywords: Hestrin-Schramm medium; bacterial cellulose; hydrocolloids; microstructure.

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References

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