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. 2023 Mar 24;9(4):e14839.
doi: 10.1016/j.heliyon.2023.e14839. eCollection 2023 Apr.

Comparison of coliform paper test and ATP bioluminescence assay for monitoring the disinfection of kitchen utensils in canteens of hebei, China

Yue Tang  1 , Jie Sun  2 , Derong Dong  1 , Xiushan Zhang  1 , Ruizhong Jia  1 , Yong Wang  1 , Yong Chen  1 , Jinpeng Guo  1 , Lianqun Jin  1
Affiliations

Comparison of coliform paper test and ATP bioluminescence assay for monitoring the disinfection of kitchen utensils in canteens of hebei, China

Yue Tang et al. Heliyon. .

Abstract

Background: Two common techniques used in canteen hygiene supervision, are the coliform paper assay, which is the standard method, and the adenosine triphosphate (ATP) bioluminescence method. The coliform paper assay requires the incubation of the sample, which is time-consuming and does not provide a real-time assessment. Meanwhile, the ATP bioluminescence assay can provide real-time kitchenware cleanliness data.

Objective: This study aimed to compare these two methods for evaluating the sanitary condition of kitchenware and explore whether the ATP bioluminescence assay can be used as a standard method in sanitary inspection.

Methods: In this study, the cluster random sampling method was used to sample kitchenware from six canteens in the Hebei province, China. Samples were, assessed through the coliform paper test and ATP bioluminescence assay.

Results: Kitchenware negative rates for the coliform paper method and the ATP test were 64.39% and 49.07%, respectively. The Escherichia coli positive detection rate grew steadily as the relative light units (RLU) value for the ATP technique increased. The kappa coefficient for the two methods was 0.549, indicating that the two methods yield relatively consistent results.

Conclusion: Although currently not considered a standard method, simply using ATP detection is advantageous for quick on-site detection in catering unit hygiene supervision.

Keywords: ATP bioluminescence; Canteen; Coliform paper test; Hygiene management; Hygiene supervision; Kitchen disinfection; Kitchen utensils disinfection.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Coliform positive rate for coliform paper test method and ATP bioluminescence assay test for various kitchen utensils.
Fig. 2
Fig. 2
Mean value of RLU of ATP bioluminescence assay test of all kinds of kitchen utensils.
Fig. 3
Fig. 3
Correlation between the ATP bioluminescence assay and coliform paper test (r = 0.864, P = 0.006).
See this image and copyright information in PMC

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