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. 2024;31(13):1740-1753.
doi: 10.2174/0929867330666230407140730.

Inflammatory Response Modulation by Low-Dose Anti-inflammatory Drugs Treatment in an In Vitro Osteoarthritis Cellular Model

Marco Iuliano  1 , Valter Santilli  2 , Andrea Mineo  2 , Marco Paoloni  2 , Paolo Rosa  1 , Giorgio Mangino  1 , Giovanna Romeo  1
Affiliations

Inflammatory Response Modulation by Low-Dose Anti-inflammatory Drugs Treatment in an In Vitro Osteoarthritis Cellular Model

Marco Iuliano et al. Curr Med Chem. 2024.

Abstract

Background: Low-dose-medicine is based on the administration of low doses of biological regulators to restore the immunologic balance altered in the disease. Cytokines are pivotal regulators of cellular and tissue functions and impaired crosstalk, due to an imbalance between specific cytokines, it is fundamental in acute inflammation and diseases correlated to low-grade chronic inflammation. Osteoarthritis is the most prevalent arthritic disease and a leading cause of disability. In the treatment of muscle- skeletal pathologies, the therapeutic integration of conventional medicine with homotoxicology, or low-dose-medicine appears to be beneficial.

Objective: This study aims to get more insights into the role of inflammatory cytokines and chemokines during the development of osteoarthritis and to evaluate a possible blocking strategy using anti-inflammatory molecules, we resort to an in vitro experimental model using an established human chondrosarcoma cell line that underwent to a well known pro-inflammatory stimulus as bacterial lipopolysaccharide.

Methods: We tested the production of inflammatory-related cytokines and chemokines, and the efficacy of low-dose (LD) administration of anti-inflammatory compounds, namely IL-10 and anti-IL-1, to block inflammatory cellular pathways.

Results: Following an inflammatory insult, chondrocytes upregulated the expression of several pro-inflammatory cyto-/chemokines and this induction could be counteracted by LD IL-10 and anti-IL-1. We reported that these effects could be ascribed to an interfering effect of LD drugs with the NF-κB signaling.

Conclusion: Our results provided a good indication that LD drugs can be effective in inhibiting the inflammatory response in chondrocytes opening the way to new therapies for the treatment of diseases such as osteoarthritis.

Keywords: NF- κB.; Osteoarthritis; anti-IL-1; chemokines; interleukin-10; low grade inflammation; low-dose-medicine.

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References

    1. Robinson W.H.; Lepus C.M.; Wang Q.; Raghu H.; Mao R.; Lindstrom T.M.; Sokolove J.; Low-grade inflammation as a key mediator of the pathogenesis of osteoarthritis. Nat Rev Rheumatol 2016,12(10),580-592 - DOI - PubMed
    1. Mabey T.; Honsawek S.; Cytokines as biochemical markers for knee osteoarthritis. World J Orthop 2015,6(1),95-105 - DOI - PubMed
    1. Kapoor M.; Martel-Pelletier J.; Lajeunesse D.; Pelletier J.P.; Fahmi H.; Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011,7(1),33-42 - DOI - PubMed
    1. Aman Z.S.; DePhillipo N.N.; Familiari F.; Dickens J.F.; LaPrade R.F.; Dekker T.J.; Acute intervention with selective interleukin-1 inhibitor therapy may reduce the progression of posttraumatic osteoarthritis of the knee: A systematic review of current evidence. Arthroscopy 2022,38(8),2543-2556 - DOI - PubMed
    1. Li M.; Li H.; Ran X.; Yin H.; Luo X.; Chen Z.; Effects of adenovirus-mediated knockdown of IRAK4 on synovitis in the osteoarthritis rabbit model. Arthritis Res Ther 2021,23(1),294 - DOI - PubMed

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