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. 2023 Apr 11;9(1):39.
doi: 10.1038/s41421-023-00530-6.

Dioxygenase JID1 mediates the modification of OPDA to regulate jasmonate homeostasis

Rong Yi  1   2 , Ran Du  3 , Jiaojiao Wang  1 , Jijun Yan  4 , Jinfang Chu  4   5 , Jianbin Yan  6 , Xiaoyi Shan  7 , Daoxin Xie  8
Affiliations

Dioxygenase JID1 mediates the modification of OPDA to regulate jasmonate homeostasis

Rong Yi et al. Cell Discov. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. JID1 modifies OPDA to regulate plant defense responses.
a Schematic of JA biosynthesis. Enzymes involved in JA biosynthesis are indicated. The enzyme(s) involved in the catalysis of OPDA is unclear. b LC-MS/MS analysis of residual OPDA and produced mo-OPDA after incubation with empty vector (top panel) or affinity-purified JID1 protein (bottom panel) respectively, monitored by UHPLC-QE/MS. RT, retention time; AA, peak area. c Relative contents of substrate OPDA and product mo-OPDA after incubation with JID1 protein. Relative content was calculated by the ratio of residual OPDA or produced mo-OPDA peak area relative to that of initial OPDA. The initial content of OPDA was set as 100%. Data are means ± SEM (n = 4). d MS/MS spectra of substrate OPDA (top panel) and product mo-OPDA (bottom panel) monitored by UHPLC-QE/MS. The m/z 77.00669 fragment of mo-OPDA is indicated in green. e JA profiles of plants without (Unwounded) or with mechanical wounding (Wounded) for 1 h. Data are means ± SEM (n = 3). Two-way ANOVA, Tukey’s post-hoc test, ns, P > 0.05, *P ≤ 0.05, **P ≤ 0.01. f, g Representative phenotypes of attacked plants (f) and S. exigua larvae (g) are shown. Scale bars, 2 cm (f), 5 mm (g). h–j Disease symptoms of plants (h), quantification of B. cinerea biomass (i), and disease severity of plants (j) are shown. Scale bar, 1 cm (h). Data are means ± SEM (n = 3). Student’s t-test, *P ≤ 0.05. dpi, days post-inoculation.
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