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. 2022 Dec 18:13:e70.
doi: 10.34172/jlms.2022.70. eCollection 2022.

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

Reza Vafaee  1 , Babak Arjmand  2 , Maryam Hamzeloo-Moghadam  3 , Zahra Razzaghi  1 , Mostafa Rezaei Tavirani  4 , Sina Rezaei Tavirani  5
Affiliations

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

Reza Vafaee et al. J Lasers Med Sci. .

Abstract

Introduction: Cold physical plasma is a growing tool in medicine which is applied for the treatment of different cancers. In the present study, the gene profiles of human melanocytes exposed to indirect cold physical plasma versus control individuals are analyzed via protein-protein interaction (PPI) network analysis. Methods: The gene expression profiles were derived from Gene Expression Omnibus (GEO), and the significant differentially expressed genes (DEGs) were decoded via "Expression Atlas". PPI network analysis was applied to find the targeted central genes by indirect cold physical plasma. Results: The main connected component of the constructed network including 74 queried DEGs and 50 added first neighbors was analyzed. Considering degree value, betweenness centrality, closeness centrality, and stress, IGF1 and HMOX1 were introduced as the central nodes. Conclusion: The finding of this study indicates that the down-regulation of IGF1 and the up-regulation of HMOX are the prominent events in response to indirect cold physical plasma treatment at the cellular level. Detection of related biological terms via gene ontology is suggested.

Keywords: Central genes; Cold physical plasma; Gene expression change; Human melanocytes; PPI network.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

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