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. 2022 Dec 13;32(6):833-845.
doi: 10.1007/s10068-022-01219-4. eCollection 2023 May.

In vivo and in vitro evaluation of stability and antioxidant activity of lycopene-nanostructured lipid carriers

Yongqiang Ma  1 , Chenchen Li  1 , Weiye Xiu  1 , Xin Wang  1
Affiliations

In vivo and in vitro evaluation of stability and antioxidant activity of lycopene-nanostructured lipid carriers

Yongqiang Ma et al. Food Sci Biotechnol. .

Abstract

This study evaluates the stability of lycopene in the presence of the prepared nanostructured lipid carriers (NLCs) under different environments and food systems and the in vitro and in vivo antioxidant activity of the lycopene nanostructured lipid carriers (Lyco-NLCs) was studied. As observed in the stability experiment, Lyco-NLCs have good storage stability within 30 days. Food additives have little effect on its stability except for metal ions. Compared with free lycopene, Lyco-NLCs showed an improved antioxidant property. In in-vitro experiments, the DPPH radical scavenging rate, hydroxyl radical scavenging capacity, and ferric reducing capacity of Lyco-NLCs increased by 90.47%, 47.43%, and 45.12%, respectively. The animal experiments showed that the activities of catalase in the kidney, superoxide dismutase in the heart, and glutathione peroxidase in the liver increased by 31.48%, 42.50%, and 21.47%, respectively. The content of malondialdehyde in serum decreased by 14.13%. The results have some significance for the practical application of lycopene.

Keywords: Antioxidant activity; Lycopene; Nanostructured lipid carrier; Stability.

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Conflict of interest statement

Conflict of interestOn behalf of all authors, the corresponding author states that there is no conflict of interest.

Figures

Fig. 1
Fig. 1
Micromorphology (A, B), particle size (C) of the Lyco-NLCs observed and recorded using the SEM, TEM and DLS technique, appearance (D), FTIR (E), and XRD (F) recorded for lycopene, Blank NLCs, and Lyco-NLCs
Fig. 2
Fig. 2
Effects of light (A), temperature (B), and freeze–thaw times (D) on the stability of Lyco-NLCs, and effect of storage on the average particle size, PDI, and Zeta potential (C) of Lyco-NLCs
Fig. 3
Fig. 3
Effect of metal ions (A), citric acid (B), sweeteners (C), thickener (D) on the stability of Lyco-NLCs
Fig. 4
Fig. 4
Results from in vitro antioxidant experiments conducted with Lyco-NLCs are shown in (A–C)
Fig. 5
Fig. 5
Results of in vivo antioxidant experiments conducted with Lyco-NLCs are shown in (A–D)
See this image and copyright information in PMC

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