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. 2023 Feb;16(2):322-328.
doi: 10.14202/vetworld.2023.322-328. Epub 2023 Feb 17.

Prevalence of Campylobacter spp. in broilers in North Lebanon

Rana Awada  1   2   3   4   5 , Ghassan Ghssein  1   2   3   4   5 , Ali El Roz  1   2   3   4   5 , Mona Farhat  2 , Nada Nehme  4 , Hussein F Hassan  5
Affiliations

Prevalence of Campylobacter spp. in broilers in North Lebanon

Rana Awada et al. Vet World. 2023 Feb.

Abstract

Background and aim: Great attention has been given recently to the prevalence of different Campylobacter spp. in poultry since the latter are considered the major contributing reservoir of human campylobacteriosis. In Lebanon, the occurrence of campylobacteriosis in humans is high. The aim of our first-of-its-kind study in the country was to estimate the prevalence of Campylobacter spp. in broilers from a convenient sample of farms in North Lebanon.

Materials and methods: One hundred twenty-five fecal samples were collected from 25 broiler farms, which were selected, examined, and classified according to their biosecurity level and rearing system. All samples were subjected to qualitative microbiological culture testing and polymerase chain reaction (PCR) assays to detect Campylobacter spp.

Results: Despite the reported use of antibiotics, cell culture and PCR were positive for 44% and 88%, respectively. This implies that this bacterium is resistant to antibiotics used on the farms. Furthermore, Campylobacter infection rate was higher in open (92%) than in closed (85%) system farms. All farms with poor biosecurity measures, and 82% of farms with good biosecurity measures had Campylobacter infections, and the difference was significant (p < 0.05).

Conclusion: Our results show that campylobacteriosis was found prevalent among broilers in North Lebanon, making them potential carriers of Campylobacter spp. Future studies should include antibiotic susceptibility testing to check the susceptibility pattern of isolates.

Keywords: Campylobacter; Lebanon; broilers; campylobacteriosis; faeco-prevalence.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure-1
Figure-1
Percentage of different classes of antibiotics used in the 25 farms.
Figure-2
Figure-2
Results of the polymerase chain reaction on agarose Gel. M=Marker, PC=Positive control, NC=Negative control, S=Sample, F=Farm.
See this image and copyright information in PMC

References

    1. Fletcher S.M, McLaws M.L, Ellis J.T. Prevalence of gastrointestinal pathogens in developed and developing countries:Systematic review and meta-analysis. J. Public Health Res. 2013;2(1):42–53. - PMC - PubMed
    1. Ibrahim J.N, Eghnatios E, El Roz A, Fardoun T, Ghssein G. Prevalence, antimicrobial resistance and risk factors for Campylobacteriosis in Lebanon. J. Infect. Dev. Ctries. 2019;13(1):11–20. - PubMed
    1. Hassan H, Salami A, Nehme N, Hakeem R.A, Hage J.E, Awada R. Prevalence and prevention of brucellosis in cattle in Lebanon. Vet. World. 2020;13(2):364–371. - PMC - PubMed
    1. Hassan H, Salami A, Ghssein G, El-Hage J, Nehme N, Awada R. Seroprevalence of Brucella abortus in cattle in Southern Lebanon using different diagnostic tests. Vet. World. 2020;13(10):2234–2242. - PMC - PubMed
    1. Wilson D, Gabriel E, Leatherbarrow A, Cheesbrough J, Gee S, Bolton E, Diggle P. Tracing the source of campylobacteriosis. PLoS Genet. 2008;4(9):e1000203. - PMC - PubMed

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