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Review
. 2023 Apr 5;12(7):1090.
doi: 10.3390/cells12071090.

Preclinical Large Animal Porcine Models for Cardiac Regeneration and Its Clinical Translation: Role of hiPSC-Derived Cardiomyocytes

Divya Sridharan  1 , Nooruddin Pracha  1 , Schaza Javed Rana  1   2 , Salmman Ahmed  1   3 , Anam J Dewani  1   4 , Syed Baseeruddin Alvi  1 , Muhamad Mergaye  1 , Uzair Ahmed  1 , Mahmood Khan  1   5
Affiliations
Review

Preclinical Large Animal Porcine Models for Cardiac Regeneration and Its Clinical Translation: Role of hiPSC-Derived Cardiomyocytes

Divya Sridharan et al. Cells. .

Abstract

Myocardial Infarction (MI) occurs due to a blockage in the coronary artery resulting in ischemia and necrosis of cardiomyocytes in the left ventricular heart muscle. The dying cardiac tissue is replaced with fibrous scar tissue, causing a decrease in myocardial contractility and thus affecting the functional capacity of the myocardium. Treatments, such as stent placements, cardiac bypasses, or transplants are beneficial but with many limitations, and may decrease the overall life expectancy due to related complications. In recent years, with the advent of human induced pluripotent stem cells (hiPSCs), newer avenues using cell-based approaches for the treatment of MI have emerged as a potential for cardiac regeneration. While hiPSCs and their derived differentiated cells are promising candidates, their translatability for clinical applications has been hindered due to poor preclinical reproducibility. Various preclinical animal models for MI, ranging from mice to non-human primates, have been adopted in cardiovascular research to mimic MI in humans. Therefore, a comprehensive literature review was essential to elucidate the factors affecting the reproducibility and translatability of large animal models. In this review article, we have discussed different animal models available for studying stem-cell transplantation in cardiovascular applications, mainly focusing on the highly translatable porcine MI model.

Keywords: cell transplantation; hiPSC-CMs; large animal model; myocardial infarction; porcine.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Stages for the translation of hiPSC-CMs from bench to bedside.
Figure 2
Figure 2
Annual number of publications involving different preclinical small and large animal models for hiPSC-CM transplantation studies (Source: Scopus).
See this image and copyright information in PMC

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