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. 2023 Jun;58(6):877-881.
doi: 10.1111/rda.14361. Epub 2023 Apr 23.

Oxidative response of sheep to transcervical applications

Nail Tekin Önder  1 , Yusuf Umut Batı  2 , Taygun Gökdemir  1 , Muhammet Can Kılıç  1 , Oğuzhan Şahin  1 , Metin Öğün  3 , Enes Akyüz  2 , Mushap Kuru  4 , Ali Haydar Kırmızıgül  2 , Savaş Yıldız  1 , Yavuz Öztürkler  1
Affiliations

Oxidative response of sheep to transcervical applications

Nail Tekin Önder et al. Reprod Domest Anim. 2023 Jun.

Abstract

This study aimed to investigate the stress-related changes that may occur in sheep following non-surgical cervical line passage. A total of 20 sheep underwent transcervical transition, and their heart rate, respiratory rate, body temperature, and blood samples were collected at specific time points: 1 h prior to the procedure, immediately after the procedure, and at 1, 2, 3, 4 and 5 h post-procedure. The study found that there was a statistically significant decrease in body temperature immediately after the procedure (p < .05); however, there were no significant differences in body temperature at other time points (p > .05). Respiratory rate remained similar throughout the study, and no significant differences were observed (p > .05). On the other hand, there was a significant increase in heart rate at the 1-h mark after the procedure (p < .05), which subsequently decreased to a similar level as the control group at the 4-h mark after the procedure (p > .05). The cortisol levels increased significantly after the application (p < .05) but decreased to a level similar to the control after 1 h (p > .05). The level of nitric oxide increased significantly in the first hour (p < .05) and decreased by the 5th hour (p < .05). There was a significant increase in malondialdehyde (MDA) and total oxidant capacity (TOC) levels 2 h after the application (p < .05), but this increase ceased at the 4th and 5th hours for MDA (p > .05) and at the 3rd, 4th and 5th hours for TOC (p > .05). The total antioxidant capacity decreased after the 2nd hour of application (p < .05) but remained similar at the fourth and 5th hours (p > .05). In conclusion, based on the cortisol data, transcervical manipulations create short-term stress. Therefore, it may be necessary to use various antioxidants before such procedures to reduce the effects of oxidative stress.

Keywords: ewe; oxidative stress; transcervical.

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