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. 2023 Jul;27(7):3937-3948.
doi: 10.1007/s00784-023-05017-y. Epub 2023 Apr 15.

Transcriptome analysis of human peri-implant soft tissue and periodontal gingiva: a paired design study

Danying Chen #  1   2   3 , Zhixin Li #  1   2   3 , Zhipeng Li  1   2   3 , Yue Sun  1   2   3 , Qifan Liu  1   2   3 , Jieting Yang  1   2   3 , Jiaying Song  1   2   3 , Huaxiong Cai  1   2   3 , Zhicai Feng  1   2   3 , Zhuofan Chen  4   5   6 , Baoxin Huang  7   8   9
Affiliations

Transcriptome analysis of human peri-implant soft tissue and periodontal gingiva: a paired design study

Danying Chen et al. Clin Oral Investig. 2023 Jul.

Abstract

Objectives: Limited information is available about the biological characterization of peri-implant soft tissue at the transcriptional level. The aim of this study was to investigate the effect of dental implant on the soft tissue in vivo by using paired samples and compare the differences between peri-implant soft tissue and periodontal gingiva at the transcriptional level.

Methods: Paired peri-implant soft tissue and periodontal gingiva tissue from 6 patients were obtained, and the pooled RNAs were analyzed by deep sequencing. Venn diagram was used to further screen out differentially expressed genes in every pair of samples. Annotation and enrichment analysis was performed. Further verification was done by quantitative real-time PCR.

Results: Totally 3549 differentially expressed genes (DEGs) were found between peri-implant and periodontal groups. The Venn diagram further identified 185 DEGs in every pair of samples, of which the enrichment analysis identified significant enrichment for cellular component was associated with external side of plasma membrane, for molecular function was protein binding, for biological process was immune system process, and for KEGG pathway was cytokine-cytokine receptor interaction. Among the DEGs, CST1, SPP1, AQP9, and SFRP2 were verified to be upregulated in peri-implant soft tissue.

Conclusions: Peri-implant soft tissue showed altered expressions of several genes related to the cell-ECM interaction compared to periodontal gingiva.

Clinical relevance: Compared to periodontal gingiva, altered cell-ECM interactions in peri-implant may contribute to the susceptibility of peri-implant diseases. At the transcriptional level, periodontal gingiva is generally considered the appropriate control for peri-implantitis, except regarding the cell-ECM interactions.

Keywords: Extracellular matrix; Peri-implant; Soft tissue; Transcriptome sequencing.

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