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. 2023 Apr 15;205(5):193.
doi: 10.1007/s00203-023-03532-w.

Low-temperature phenol-degrading microbial agent: construction and mechanism

Yu Yang  1 , Yu Zhang  2 , Cong Liu  1 , Zhiqiang Su  1 , Ruizhi Zhao  1 , Jiti Zhou  1
Affiliations

Low-temperature phenol-degrading microbial agent: construction and mechanism

Yu Yang et al. Arch Microbiol. .

Abstract

In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) was used to optimize the proportional composition of low-temperature phenol-degrading microbiota. Degradation of phenol below 160 mg L-1 by low-temperature phenol-degrading microbiota followed first-order degradation kinetics. When the phenol concentration was greater than 200 mg L-1, the overall degradation trend was in accordance with the modified Gompertz model. The experiments showed that the microbial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) could completely degrade 200 mg L-1 phenol within 36 h. The above construction method is more advantageous in bio-enhanced treatment of actual wastewater. Through the construction of microbial agents to enhance the degradation effect of phenol, it provides a feasible scheme for the biodegradation of phenol wastewater at low temperature and shows good application potential.

Keywords: Catechol-1,2-dioxygenase; Degradation kinetics; Metabolic pathways; Microbial agent.

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