Decellularized Extracellular Matrix for Tissue Engineering (Review)

doi:10.17691/stm2022.14.3.07

Review

. 2022;14(3):57-68.

doi: 10.17691/stm2022.14.3.07. Epub 2022 May 28.

Decellularized Extracellular Matrix for Tissue Engineering (Review)

E V Isaeva¹, E E Beketov², N V Arguchinskaya³, S А Ivanov⁴, P V Shegay⁵, А D Kaprin⁶

Affiliations

¹ Senior Researcher, Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
² Head of the Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
³ Junior Researcher, Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
⁴ Director; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Professor, Department of Oncology and Radiology and Nuclear Medicine; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia.
⁵ Head of the Center of Innovative Radiological and Regenerative Technologies; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia.
⁶ Professor, Academician of the Russian Academy of Sciences, General Director; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia Head of the Department of Urology and Operative Nephrology with the Course of Oncourology of the Medical Institute; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia.

PMID: 37064810
PMCID: PMC10090917
DOI: 10.17691/stm2022.14.3.07

Review

Decellularized Extracellular Matrix for Tissue Engineering (Review)

E V Isaeva et al. Sovrem Tekhnologii Med. 2022.

. 2022;14(3):57-68.

doi: 10.17691/stm2022.14.3.07. Epub 2022 May 28.

Authors

E V Isaeva¹, E E Beketov², N V Arguchinskaya³, S А Ivanov⁴, P V Shegay⁵, А D Kaprin⁶

Affiliations

¹ Senior Researcher, Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
² Head of the Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
³ Junior Researcher, Laboratory of Biomaterials and Tissue Constructions; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia.
⁴ Director; A. Tsyb Меdical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Professor, Department of Oncology and Radiology and Nuclear Medicine; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia.
⁵ Head of the Center of Innovative Radiological and Regenerative Technologies; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia.
⁶ Professor, Academician of the Russian Academy of Sciences, General Director; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia Head of the Department of Urology and Operative Nephrology with the Course of Oncourology of the Medical Institute; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia.

PMID: 37064810
PMCID: PMC10090917
DOI: 10.17691/stm2022.14.3.07

Abstract

In recent years, decellularized tissues have evolved into a new, full-fledged platform for the creation of tissue-engineered constructions. Extracellular matrix (ECM) of each tissue provides a unique tissue-specific microenvironment for resident cells with the structure and biochemical signaling required for their functioning. The decellularized ECM (dECM) has been established to influence cell differentiation. The review provides recent data on the composition and functions of the ECM, methods for obtaining decellularized tissues, and their application in tissue engineering depending on their physical form (scaffold, powder, or hydrogel). The effect of the matrix source, decellularization and sterilization techniques on dECM composition has been considered. Regulatory mechanisms of cell differentiation by the extracellular matrix are discussed. Differences in the protein composition of the native and decellularized materials are presented. Application of dECM in the bioink composition for regeneration of various tissues using bioprinting technologies is also considered. It has been concluded that successful application of dECM in tissue engineering and regenerative medicine requires a permanent and biologically suitable dECM source, optimized tissue decellularization protocols, improved mechanical properties of dECM-derived bioinks, and prevention of immunological reaction of the organism.

Keywords: dECM; decellularization; decellularized extracellular matrix; extracellular matrix remodeling; tissue engineering.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest. The authors have no conflicts of interest to declare.

Cited by

Bioactive decellularized extracellular matrix-based hydrogel supports human adipose tissue-derived stem cell maintenance and fibrocartilage phenotype.
Josino R, Stimamiglio MA. Josino R, et al. Front Bioeng Biotechnol. 2024 Jan 8;11:1304030. doi: 10.3389/fbioe.2023.1304030. eCollection 2023. Front Bioeng Biotechnol. 2024. PMID: 38260748 Free PMC article.
Decellularized tissues as platforms for digestive system cancer models.
Seifi Z, Khazaei M, Cheraghali D, Rezakhani L. Seifi Z, et al. Heliyon. 2024 May 21;10(11):e31589. doi: 10.1016/j.heliyon.2024.e31589. eCollection 2024 Jun 15. Heliyon. 2024. PMID: 38845895 Free PMC article. Review.
Cartilage Laser Engraving for Fast-Track Tissue Engineering of Auricular Grafts.
Kisel AA, Stepanov VA, Isaeva EV, Demyashkin GA, Isaev EI, Smirnova EI, Yatsenko EM, Afonin GV, Ivanov SA, Atiakshin DA, Shegay PV, Kaprin AD, Klabukov ID, Baranovskii DS. Kisel AA, et al. Int J Mol Sci. 2024 Oct 27;25(21):11538. doi: 10.3390/ijms252111538. Int J Mol Sci. 2024. PMID: 39519090 Free PMC article.
Decellularized diseased tissues: current state-of-the-art and future directions.
Li X, Shan J, Chen X, Cui H, Wen G, Yu Y. Li X, et al. MedComm (2020). 2023 Nov 20;4(6):e399. doi: 10.1002/mco2.399. eCollection 2023 Dec. MedComm (2020). 2023. PMID: 38020712 Free PMC article. Review.
Engineering the Future of Regenerative Medicines in Gut Health with Stem Cell-Derived Intestinal Organoids.
Kumar D, Gupta S, Gupta V, Tanwar R, Chandel A. Kumar D, et al. Stem Cell Rev Rep. 2025 Jun;21(5):1449-1470. doi: 10.1007/s12015-025-10893-w. Epub 2025 May 17. Stem Cell Rev Rep. 2025. PMID: 40380985 Review.

See all "Cited by" articles

References

1. Beketov E.E., Isaeva E.V., Shegay P.V., Ivanov S.A., Kaprin A.D. Current state of tissue engineering for cartilage regeneration. Geny i kletki. 2019;14(2):12–20. doi: 10.23868/201906013. - DOI
1. Agmon G., Christman K.L. Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Curr Opin Solid State Mater Sci. 2016;20(4):193–201. doi: 10.1016/j.cossms.2016.02.001. - DOI - PMC - PubMed
1. Hoshiba T., Lu H., Kawazoe N., Chen G. Decellularized matrices for tissue engineering. Expert Opin Biol Ther. 2010;10(12):1717–1728. doi: 10.1517/14712598.2010.534079. - DOI - PubMed
1. Frantz C., Stewart K.M., Weaver V.M. The extracellular matrix at a glance. J Cell Sci. 2010;123(Pt 24):4195–4200. doi: 10.1242/jcs.023820. - DOI - PMC - PubMed
1. Clause K.C., Barker T.H. Extracellular matrix signaling in morphogenesis and repair. Curr Opin Biotechnol. 2013;24(5):830–833. doi: 10.1016/j.copbio.2013.04.011. - DOI - PMC - PubMed

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- Europe PubMed Central
- PubMed Central

[1] Beketov E.E., Isaeva E.V., Shegay P.V., Ivanov S.A., Kaprin A.D. Current state of tissue engineering for cartilage regeneration. Geny i kletki. 2019;14(2):12–20. doi: 10.23868/201906013. - DOI

[2] Beketov E.E., Isaeva E.V., Shegay P.V., Ivanov S.A., Kaprin A.D. Current state of tissue engineering for cartilage regeneration. Geny i kletki. 2019;14(2):12–20. doi: 10.23868/201906013. - DOI

[3] Agmon G., Christman K.L. Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Curr Opin Solid State Mater Sci. 2016;20(4):193–201. doi: 10.1016/j.cossms.2016.02.001. - DOI - PMC - PubMed

[4] Agmon G., Christman K.L. Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Curr Opin Solid State Mater Sci. 2016;20(4):193–201. doi: 10.1016/j.cossms.2016.02.001. - DOI - PMC - PubMed

[5] Hoshiba T., Lu H., Kawazoe N., Chen G. Decellularized matrices for tissue engineering. Expert Opin Biol Ther. 2010;10(12):1717–1728. doi: 10.1517/14712598.2010.534079. - DOI - PubMed

[6] Hoshiba T., Lu H., Kawazoe N., Chen G. Decellularized matrices for tissue engineering. Expert Opin Biol Ther. 2010;10(12):1717–1728. doi: 10.1517/14712598.2010.534079. - DOI - PubMed

[7] Frantz C., Stewart K.M., Weaver V.M. The extracellular matrix at a glance. J Cell Sci. 2010;123(Pt 24):4195–4200. doi: 10.1242/jcs.023820. - DOI - PMC - PubMed

[8] Frantz C., Stewart K.M., Weaver V.M. The extracellular matrix at a glance. J Cell Sci. 2010;123(Pt 24):4195–4200. doi: 10.1242/jcs.023820. - DOI - PMC - PubMed

[9] Clause K.C., Barker T.H. Extracellular matrix signaling in morphogenesis and repair. Curr Opin Biotechnol. 2013;24(5):830–833. doi: 10.1016/j.copbio.2013.04.011. - DOI - PMC - PubMed

[10] Clause K.C., Barker T.H. Extracellular matrix signaling in morphogenesis and repair. Curr Opin Biotechnol. 2013;24(5):830–833. doi: 10.1016/j.copbio.2013.04.011. - DOI - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Decellularized Extracellular Matrix for Tissue Engineering (Review)

Affiliations

Decellularized Extracellular Matrix for Tissue Engineering (Review)

Authors

Affiliations

Abstract

Conflict of interest statement

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources