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. 2022 Dec 5;15(1):110-116.
doi: 10.1055/s-0042-1757234. eCollection 2023 Mar.

Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Immunochromatography for Rotavirus and Adenovirus Detection in Children below Five Years with Acute Gastroenteritis

Smitha Bagali  1 , Laxmi Kakhandaki  1 , Rashmi Karigoudar  1 , Sanjay Wavare  1 , Praveen R Shahapur  1 , Mallanagouda M Patil  2
Affiliations

Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Immunochromatography for Rotavirus and Adenovirus Detection in Children below Five Years with Acute Gastroenteritis

Smitha Bagali et al. J Lab Physicians. .

Abstract

Introduction The most frequent etiologies of viral gastroenteritis among young children are rotavirus and enteric adenovirus. The clinical signs and symptoms of viral gastroenteritis are not distinct enough to allow for diagnosis. For the diagnosis and treatment of acute gastroenteritis, it is preferable to use quick, simple, and low-cost procedures. This study was undertaken to determine efficacy of immune-chromatography test (ICT) in comparison with enzyme-linked immunosorbent assay (ELISA) to detect rotavirus and adenovirus antigen in fecal specimen among children less than 5 years of age with acute gastroenteritis. Materials and Methods In a cross-sectional observational study, 314 fecal samples were collected from children aged less than 5 years with acute gastroenteritis attending or admitted to a tertiary care hospital during the 1 year study period. Samples were tested for rotavirus and adenovirus antigen using ICT and ELISA. Results Among the 314 children evaluated, 112 (35.66%) had rotavirus infection, nine (2.86%) had adenovirus infection, and three (0.95%) had both rotavirus and adenovirus infection. This study found that ICT is 98.20% sensitive and 100% specific for the diagnosis of rotaviral diarrhea and 100% sensitive and 99.7% specific for adenovirus diarrhea, compared to ELISA. Conclusion Immunochromatography tests used for the detection of rotavirus and adenovirus in the fecal sample showed a high degree of sensitivity and specificity. The ICT is easy to perform and rapid, and it does not require any special equipment. Hence, the ICT could be used as an alternative method for detecting viral pathogens in clinical practice.

Keywords: ELISA; ICT; adenovirus; diarrhea; rotavirus.

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Conflict of interest statement

Conflict of Interest None declared.

Figures

Fig. 1
Fig. 1
Month-wise distribution of number of rotavirus and adenovirus-positive children with age less than 5 years.
See this image and copyright information in PMC

References

    1. Diarrhoeal disease Who.intPublished 2021. Accessed June 13, 2022https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
    1. Diarrhoea - UNICEF DATA UNICEF DATAPublished 2021. Accessed June 13, 2022https://data.unicef.org/topic/child-health/diarrhoeal-disease/
    1. Lakshminarayanan S, Jayalakshmy R. Diarrheal diseases among children in India: current scenario and future perspectives. J Nat Sci Biol Med. 2015;6(01):24–28. - PMC - PubMed
    1. GBD 2016 Diarrhoeal Disease Collaborators . Estimates of the global, regional, and national morbidity, mortality, and aetiologies of diarrhoea in 195 countries: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Infect Dis. 2018;18(11):1211–1228. - PMC - PubMed
    1. GBD Diarrhoeal Diseases Collaborators Diarrhoeal Diseases Collaborators G BD.Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015[published correction appears in Lancet Infect Dis. 2017 Sep;17(9):897]Lancet Infect Dis 20171709909–948. - PMC - PubMed