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. 2023;29(5):2021-2029.
doi: 10.1007/s11581-023-04944-w. Epub 2023 Mar 9.

Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus

Qiwen Bao  1 , Gang Li  1 , Zhengchun Yang  2 , Jun Liu  2 , Hanjie Wang  3 , Gaoju Pang  3 , Qianjin Guo  4 , Jun Wei  5 , Wenbo Cheng  6 , Ling Lin  1
Affiliations

Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A virus

Qiwen Bao et al. Ionics (Kiel). 2023.

Abstract

To cope with the easy transmissibility of the avian influenza A virus subtype H1N1, a biosensor was developed for rapid and highly sensitive electrochemical immunoassay. Based on the principle of specific binding between antibody and virus molecules, the active molecule-antibody-adapter structure was formed on the surface of an Au NP substrate electrode; it included a highly specific surface area and good electrochemical activity for selective amplification detection of the H1N1 virus. The electrochemical test results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode was used for the electrochemical detection of the H1N1 virus with a sensitivity of 92.1 µA (pg/mL)-1 cm2, LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of (R 2 = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for the molecular detection of the H1N1 virus will be of great use in the field of epidemic prevention and raw poultry protection.

Supplementary information: The online version contains supplementary material available at 10.1007/s11581-023-04944-w.

Keywords: Au NPs; H1N1 virus; Immunoassay; Virus sensor.

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Conflict of interest statement

Conflict of interestThe authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Fabrication of the BSA/H1N1 Ab/Glu/Cys/Au NP/CP immunosensor
Fig. 2
Fig. 2
XRD patterns of Au NPs
Fig. 3
Fig. 3
a SEM image and b, c the image right are the distribution of C and Au element corresponding to a
Fig. 4
Fig. 4
Differential pulse voltammetry (DPV) curves recorded by the CP (a), Au NP/CP (b), Cys/Au NP/CP (c), Glu/Cys/Au NP/CP (d), and H1N1 Ab/Glu/Cys/Au NP/CP (e) in PBS (0.01 M)
Fig. 5
Fig. 5
Cycle voltammetry (CV) curves recorded by the Glu/Cys/Au NP/CP, H1N1 Ab/Glu/Cys/Au NP/CP and BSA/H1N1 Ab/Glu/Cys/Au NP/CP in PBS (0.01 M) containing 5 ng/ml H1N1 antigen
Fig. 6
Fig. 6
DPV curve recorded by Au NP/CP, Cys/Au NP/CP, Glu/Cys/Au NP/CP, and H1N1 Ab/Glu/Cys/Au NP/CP DPV in PBS (0.01 M) containing 0, 0.25, and 5 pg/mL of H1N1 antigen, respectively
Fig. 7
Fig. 7
Column diagram of CP (a), Au NP/CP (b), Cys/Au NP/CP (c), Glu/Cys/Au NP/CP (d), and H1N1 Ab/Glu/Cys/Au NP/CP (e) drawn according to the peak current response of the selected DPV line and the corresponding concentration
Fig. 8
Fig. 8
a DPV curves obtained from BSA/H1N1 Ab/Glu/Cys/Au NP/CP composite electrode in the presence of PBS (0.01 M) containing 0–5 pg/mL of H1N1 antigen; b linear fitting curve
See this image and copyright information in PMC

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