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. 2023:2662:125-133.
doi: 10.1007/978-1-0716-3167-6_11.

Calculating Diet-Induced Thermogenesis in Mice

Tomomi Yamazaki  1
Affiliations

Calculating Diet-Induced Thermogenesis in Mice

Tomomi Yamazaki. Methods Mol Biol. 2023.

Abstract

Diet-induced thermogenesis (DIT) is the increase in energy expenditure (EE) associated with food intake. Increasing DIT may lead to weight loss, so it is expected that increasing DIT will decrease body mass index and body fat mass. DIT in humans has been measured in various ways, but there is no way to calculate absolute DIT values in mice. Therefore, we developed a method to measure DIT in mice by applying a method more commonly used in humans. First, we measure the energy metabolism of mice under fasting conditions. EE is then plotted against the square root of activity, and a linear regression equation is fitted to the data. Next, we measure the energy metabolism of mice fed ad libitum and plotted EE in the same way. DIT is calculated by subtracting the predicted EE value from the EE value of mice fed at the same activity count. This method not only allows observation of the time course of the absolute value of DIT but also allows calculation of the ratio of DIT to caloric intake and the ratio of DIT to EE.

Keywords: Diet-induced thermogenesis; Energy expenditure; Fasting; Feeding; Physical activity; Regression equation.

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References

    1. Tappy L (1996) Thermic effect of food and sympathetic nervous system activity in humans. Reprod Nutr Dev 36(4):391–397. https://doi.org/10.1051/rnd:19960405 - DOI - PubMed
    1. Lam YY, Ravussin E (2016) Analysis of energy metabolism in humans: a review of methodologies. Mol Metab 5(11):1057–1071. https://doi.org/10.1016/j.molmet.2016.09.005 - DOI - PubMed - PMC
    1. Westerterp KR (2004) Diet induced thermogenesis. Nutr Metab (Lond) 1(1):5. https://doi.org/10.1186/1743-7075-1-5 - DOI - PubMed
    1. Ravussin E, Lillioja S, Anderson TE, Christin L, Bogardus C (1986) Determinants of 24-hour energy expenditure in man. Methods and results using a respiratory chamber. J Clin Invest 78(6):1568–1578. https://doi.org/10.1172/JCI112749 - DOI - PubMed - PMC
    1. Segal KR, Chun A, Coronel P, Cruz-Noori A, Santos R (1992) Reliability of the measurement of postprandial thermogenesis in men of three levels of body fatness. Metabolism 41(7):754–762. https://doi.org/10.1016/0026-0495(92)90316-3 - DOI - PubMed

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