Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2662:209-217.
doi: 10.1007/978-1-0716-3167-6_19.

Isolation of Adipose Tissue Extracellular Vesicles

Jessica M Sabio  1 , Clair Crewe  2   3
Affiliations

Isolation of Adipose Tissue Extracellular Vesicles

Jessica M Sabio et al. Methods Mol Biol. 2023.

Abstract

Extracellular vesicles (EVs) produced by adipocytes and other adipose tissue (AT) cells are present in the space between cells in the tissue and in circulation. These EVs have been shown to robustly signal between cells in the tissue and in distal organs. AT has unique biophysical properties that require an optimized EV isolation protocol that ensures an uncontaminated EV isolate. This protocol can be used to isolate and characterize the total, heterogeneous population of EVs from the AT.

Keywords: Adipocyte; Adipose tissue; Exosome; Extracellular vesicle; Metabolism.

PubMed Disclaimer

References

    1. Crewe C, Scherer PE (2022) Intercellular and interorgan crosstalk through adipocyte extracellular vesicles. Rev Endocr Metab Disord 23(1):61–69. https://doi.org/10.1007/s11154-020-09625-x - DOI - PubMed
    1. Flaherty SE 3rd, Grijalva A, Xu X, Ables E, Nomani A, Ferrante AW Jr (2019) A lipase-independent pathway of lipid release and immune modulation by adipocytes. Science 363(6430):989–993. https://doi.org/10.1126/science.aaw2586 - DOI - PubMed - PMC
    1. Thery C, Witwer KW, Aikawa E et al (2018) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7(1):1535750. https://doi.org/10.1080/20013078.2018.1535750 - DOI - PubMed - PMC
    1. Rider MA, Hurwitz SN, Meckes DG Jr (2016) ExtraPEG: a polyethylene glycol-based method for enrichment of extracellular vesicles. Sci Rep 6:23978. https://doi.org/10.1038/srep23978 - DOI - PubMed - PMC

LinkOut - more resources