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. 2023 Jun;54(2):1295-1301.
doi: 10.1007/s42770-023-00972-w. Epub 2023 Apr 20.

Detection of G3 human-like rotavirus in institutionalized dogs from Brazil

Macyclelma Alves Albuquerque  1 , Danielle Rodrigues de Deus  1 , Patrícia Santos Lobo  2 , Dielle Monteiro Teixeira  2 , Marcelino Antonio Costa Maués  3 , Jedson Ferreira Cardoso  2 , Luciana Damascena da Silva  2 , Yvone Benchimol Gabbay  2 , Hugo Reis Resque  2 , Luana da Silva Soares  2 , Jones Anderson Monteiro Siqueira  2 , Sylvia Fátima Santos Guerra  4
Affiliations

Detection of G3 human-like rotavirus in institutionalized dogs from Brazil

Macyclelma Alves Albuquerque et al. Braz J Microbiol. 2023 Jun.

Abstract

Viral gastroenteritis is a common clinical problem in dogs and group A rotavirus (RVA) is one of the agents involved in this etiology. It mainly affects dogs in the first 6 months of life, and these animals are considered an important reservoir and potential transmitters of the virus to other susceptible hosts, such as humans. Among the different types of RVA, G3 is the most detected in dogs, and this genotype is also involved in infections in other animals, including humans. Thus, the present study aims to investigate the presence of RVA in samples of dogs from a public kennel. A total of 64 fecal samples from dogs with diarrhea were analyzed, collected from April 2019 to March 2020, from the kennel of the Zoonosis Control Center, located in Belém, a city in the North of Brazil. The extracted genetic material was subjected to reverse transcription followed by real-time PCR (RT-qPCR); the positives were tested by RT-PCR with a specific primer for the RVA VP7 gene, after nucleotide sequencing and phylogenetic analysis. One sample was subjected to high-performance sequencing. A positivity of 7.8% (5/64) was observed for RVA, all characterized as G3, grouping in the G3-III lineage, with greater similarity to human samples. Different regions of the RVA genome fragments were found. These results emphasize the need for animal health surveillance to better understand the global strain dispersion of RVA and elucidate possible interspecies transmission events, monitoring the genetic diversity of this pathogen.

Keywords: Canine; Diarrhea; Gastroenteritis; Zoonosis.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Phylogenetic relationship of samples CAN2059 and CAN2060 (highlighted in gray) with other RVA sequences from Brazil and the world, based on the nucleotide sequence of the VP7 gene from G3 samples. It was using the maximum likelihood method of the FastTree software, including the GTR + Gamma nucleotide substitution model + F. The values shown (> 70%) indicate support for 1000 bootstrap values
Fig. 2
Fig. 2
Viral RVA categorization of CAN2060 strain readings compared in the viral protein database (BlastX, e-value 10−2) and graphically demonstrated by the Krona Chats tools
See this image and copyright information in PMC

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