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. 2023 Apr 14:16:2203-2216.
doi: 10.2147/IDR.S398951. eCollection 2023.

Phenotypic Characterization and Antibiograms of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated at the Human-Animal-Environment Interface Using a One Health Approach Among Households in Wakiso District, Uganda

James Muleme  1   2 , Clovice Kankya  2 , Musso Munyeme  3 , David Musoke  1 , John C Ssempebwa  1 , John Bosco Isunju  1 , Rogers Wambi  2   4 , Bonny Enock Balugaba  1 , Tahalu Sekulima  5 , Richard K Mugambe  1 , Simeon Cadmus  6 , Henry M Kajumbula  7
Affiliations

Phenotypic Characterization and Antibiograms of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolated at the Human-Animal-Environment Interface Using a One Health Approach Among Households in Wakiso District, Uganda

James Muleme et al. Infect Drug Resist. .

Abstract

Background: The occurrence of extended spectrum beta-lactamase (ESBL) producing bacteria such as Escherichia coli has increasingly become recognized beyond hospital settings. Resistance to other types of antibiotics limits treatment options while the existence of such bacteria among humans, animals, and the environment is suggestive of potential zoonotic and reverse-zoonotic transmission. This study aimed to establish the antibiotic susceptibility profiles of the ESBL-producing Escherichia coli (ESBL-EC) from human, animal, and environmental isolates obtained among farming households within Wakiso district using a One Health approach.

Methods: A total of 100 ESBL-EC isolates from humans 35/100 (35%), animals 56/100 (56%), and the environment 9/100 (9%) were tested for susceptibility to 11 antibiotics. This was done using the Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Data were analyzed in STATA ver. 16 and graphs were drawn in Microsoft excel ver. 10.

Results: Most of the ESBL-EC isolates (98%) were resistant to more than two antibiotics. ESBL-EC isolates were most susceptible to meropenem (MEM) (88.0%), and imipenem (82.0%) followed by gentamicin (72%). ESBL-EC isolates from humans were most susceptible to meropenem (MEM) followed by imipenem (IPM)> gentamicin (CN)> ciprofloxacin (CIP). Animal samples were more susceptible to MEM, IPM, and CN but were highly resistant to cefotaxime (CTX)> cefepime (FEP)>other antibiotics. Multidrug resistance (MDR) was mostly reported among households keeping goats under intensive husbandry practices. Seven percent of the isolates exhibited carbapenem resistance while 22% showed aminoglycoside resistance. Similar resistance patterns among humans, animals, and environmental samples were also reported.

Conclusion: Our study provides baseline information on non-hospital-based MDR caused by ESBL-EC using a One Health approach. ESBL-EC isolates were prevalent among apparently healthy community members, animals, and their environment. It is important to conduct more One Health approach studies to generate evidence on the drivers, resistance patterns, and transmission of ESBL-producing organisms at the human-animal-environmental interface.

Keywords: ESBL-producing; Escherichia coli; Wakiso; antibiogram; community; one health.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Primary cultures and subcultures.
Figure 2
Figure 2
Modified double disc diffusion test results.
Figure 3
Figure 3
Antibiograms of ESBL-EC Human isolates.
Figure 4
Figure 4
Antibiograms of ESBL-EC Environmental isolates.
Figure 5
Figure 5
Antibiograms of ESBL-EC Animal isolates.
Figure 6
Figure 6
Antibiotic sensitivity profiles for isolates from four unique households.
See this image and copyright information in PMC

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References

    1. Ferri M, Ranucci E, Romagnoli P, Giaccone V. Antimicrobial resistance: a global emerging threat to public health systems. Crit Rev Food Sci Nutr. 2017;57:2857–2876. doi:10.1080/10408398.2015.1077192 - DOI - PubMed
    1. Bassetti M, Poulakou G, Ruppe E, et al. Antimicrobial resistance in the next 30 years, humankind, bugs and drugs: a visionary approach. Intensive Care Med. 2017;43:1464–1475. doi:10.1007/s00134-017-4878-x - DOI - PubMed
    1. Patel A. Tackling antimicrobial resistance in the shadow of covid-19. MBio. 2021;12. doi:10.1128/mBio.00473-21 - DOI - PMC - PubMed
    1. Cassini A, Högberg LD, Plachouras D, et al. Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: a population-level modelling analysis. Lancet Infect Dis. 2019;19:56–66. doi:10.1016/S1473-3099(18)30605-4 - DOI - PMC - PubMed
    1. World Health Organization. WHO Report on Surveillance of Antibiotic Consumption: 2016–2018 Early Implementation. Geneva: World Health Organization; 2018.