Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct-Dec;26(4):596.
doi: 10.4103/jomfp.jomfp_49_21. Epub 2022 Dec 22.

Preoperative platelet-lymphocyte ratio and neutrophil-lymphocyte ratio as predictors of occult lymph node metastasis detected using Desmoglein 3 and Cytokeratin in Indian population

Aadithya B Urs  1 , Jeyaseelan Augustine  1 , Nita Khurana  2 , Akanksha Uniyal  1 , J C Passey  3 , Ravi Meher  3
Affiliations

Preoperative platelet-lymphocyte ratio and neutrophil-lymphocyte ratio as predictors of occult lymph node metastasis detected using Desmoglein 3 and Cytokeratin in Indian population

Aadithya B Urs et al. J Oral Maxillofac Pathol. 2022 Oct-Dec.

Abstract

Aim: This study aims to assess whether preoperative platelet-lymphocyte ratio (PLR) and neutrophil-lymphocyte ratio (NLR) can predict occult metastasis in oral squamous cell carcinoma (OSCC).

Materials and methods: Thirty-five OSCC cases were analyzed for clinicopathological and hematological data. Cases without metastasis (pN0) were checked for micrometastasis immunohistochemically using Desmoglein 3 (DSG3) and Cytokeratin (CK). Mean PLR and NLR were compared and analyzed between the study groups.

Results: Metastatic deposits were detected in 9 out of 26 pN0 cases (34.6%) accounting for 11 out of 62 (17%) lymph nodes subjected to immunohistochemistry. The mean PLR was higher in OSCC cases with or without occult metastasis in comparison to controls (P < 0.001). No significant difference was found in the mean PLR and NLR between OSCC cases with and without occult metastasis. Furthermore, we found DSG3+ sinus histiocytes within the lymph nodes in majority of cases which is least reported in literature.

Conclusion: A significant percentage of cases showed occult metastasis in this study which led to upstaging of tumor. Although PLR was elevated in OSCC cases, it did not have a positive correlation with the presence of occult metastasis but was able to successfully distinguish OSCC patients from healthy individuals.

Keywords: Occult metastasis; oral squamous cell carcinoma; platelet lymphocyte ratio.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Photomicrograph showing immunopositivity in macrometastatic deposit on staining with (a) DSG3 (IHC, 10x) and, (b) CK (IHC, 10x), (c) Photomicrograph of another case showing presence of ITC on immunostaining with DSG3 (IHC, 40x). Inset showing positivity with CK within the same cells (IHC, 40x), (d) Photomicrograph showing presence of ITC (Left, HE, 40x). Positive immunostaining noted with CK (Right, IHC, 40x), but negative staining was noted with DSG3 (Middle, IHC, 40x)
Figure 2
Figure 2
Photomicrograph showing normal oral epithelium stained with, (a) DSG3 (IHC, 40x), (b) CK (IHC, 40x)
Figure 3
Figure 3
Photomicrograph showing granular positivity in sinus histiocytes on immunostaining with DSG3 (Left, IHC, 40x) and CK (Right, IHC, 40x)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 2009;45:309–16. - PubMed
    1. Shenoi R, Devrukhkar V, Chaudhuri, Sharma BK, Sapre SB, Chikhale A. Demographic and clinical profile of oral squamous cell carcinoma patients: A retrospective study. Indian J Cancer. 2012;49:21–6. - PubMed
    1. Roxburgh CS, McMillan DC. Cancer and systemic inflammation: Treat the tumour and treat the host. Br J Cancer. 2014;110:1409–12. - PMC - PubMed
    1. McMillan DC. Systemic inflammation, nutritional status and survival in patients with cancer. Curr Opin Clin Nutr Metab Care. 2009;12:223–6. - PubMed
    1. Pedrazzani C, Mantovani G, Fernandes E, Bagante F, Luca Salvagno G, Surci N, et al. Assessment of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and platelet count as predictors of long-term outcome after R0 resection for colorectal cancer. Sci Rep. 2017;7:1494. - PMC - PubMed