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. 1979 Feb;76(2):575-9.
doi: 10.1073/pnas.76.2.575.

Nucleotide sequence of Escherichia coli K-12 replication origin

K Sugimoto, A Oka, H Sugisaki, M Takanami, A Nishimura, Y Yasuda, Y Hirota

Nucleotide sequence of Escherichia coli K-12 replication origin

K Sugimoto et al. Proc Natl Acad Sci U S A. 1979 Feb.

Abstract

From subfragments of an EcoRI fragment (9 kilobase pairs) that contained the replication origin of the Escherichia coli chromosome and had been cloned as a recombinant with a nonreplicating DNA fragment coding for ampicillin resistance, small derivative plasmids were constructed. The smallest of these, pTSO151, contained a segment of 463 base pairs as the chromosomal component. Another plasmid, pSY134, constructed from BamHI digests of the EcoRI fragment and mini-F(pMF21), contained a region of 422 base pairs identical with a corresponding region in pTSO151. We conclude that the replication origin of E. coli chromosome is located within this 422-base-pair segment. The nucleotide sequence of this segment is presented.

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References

    1. Nucleic Acids Res. 1977 Jul;4(7):2213-22 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Feb;76(2):580-4 - PubMed
    1. Plasmid. 1977 Nov;1(1):67-77 - PubMed
    1. Mol Gen Genet. 1978 Apr 17;160(3):287-95 - PubMed
    1. Gene. 1977;2(2):95-113 - PubMed

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