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. 2023 Jun;50(6):4975-4982.
doi: 10.1007/s11033-023-08391-8. Epub 2023 Apr 22.

PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages

Lucie Ballonová  1   2 , Petra Kulíšková  1   3 , Peter Slanina  3 , Julie Štíchová  3 , Marcela Vlková  3 , Roman Hakl  3 , Jiří Litzman  3 , Přemysl Souček  4   5 , Tomáš Freiberger  1   3
Affiliations

PLAUR splicing pattern in hereditary angioedema patients' monocytes and macrophages

Lucie Ballonová et al. Mol Biol Rep. 2023 Jun.

Abstract

Background: The PLAUR gene encodes the urokinase-like plasminogen activator receptor (uPAR) and may undergo alternative splicing. Excluding cassette exons 3, 5 and 6 from the transcript results in truncated protein variants whose precise functions have not been elucidated yet. The PLAUR gene is one of several expressed in myeloid cells, where uPAR participates in different cellular processes, including the contact activation system and kallikrein-kinin system, which play an important role in hereditary angioedema (HAE) pathogenesis. A hypothesis about the PLAUR splicing pattern impact on HAE severity was tested.

Methods and results: The RT-PCR quantified by capillary electrophoresis was used. Although no significant difference in alternative transcript frequency was observed between healthy volunteers and HAE patients, a significant increase in all cassette exon inclusion variants was revealed during monocyte-to-macrophage differentiation.

Conclusions: PLAUR alternative splicing in monocytes and macrophages neither was different between HAE patients and healthy controls, nor reflected disease severity. However, the results showed an PLAUR splicing pattern was changing during monocyte-to-macrophage differentiation, but the significance of these changes is unknown and awaits future clarification.

Keywords: Differentiation; HAE; Macrophages; Monocyte; PLAUR; uPAR.

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References

    1. Pyke C, Eriksen J, Solberg H et al (1993) An alternatively spliced variant of mRNA for the human receptor for urokinase plasminogen activator. FEBS Lett 326:69–74. https://doi.org/10.1016/0014-5793(93)81763-P - DOI - PubMed
    1. Lv T, Zhao Y, Jiang X et al (2021) UPAR: an essential factor for tumor development. J Cancer 12:7026–7040. https://doi.org/10.7150/jca.62281 - DOI - PubMed - PMC
    1. Smith HW, Marshall CJ (2010) Regulation of cell signalling by uPAR. Nat Rev Mol Cell Biol 11:23–36. https://doi.org/10.1038/nrm2821 - DOI - PubMed
    1. Kjaergaard M (2008) Structure and ligand interactions of the urokinase receptor (uPAR). Front Biosci 13:5441–5461. https://doi.org/10.2741/3092 - DOI - PubMed
    1. Degryse B, Resnati M, Czekay R-P et al (2005) Domain 2 of the Urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity. J Biol Chem 280:24792–24803. https://doi.org/10.1074/jbc.M413954200 - DOI - PubMed

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