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. 2023 Apr 5:14:1118853.
doi: 10.3389/fmicb.2023.1118853. eCollection 2023.

Gerhardtia tomentosa and Ossicaulis borealis (Agaricales, Lyophyllaceae)-Two new species from northeast China

Yue Qi  1 , Fei Xu  1 , Zhao-Qian Yang  1 , Jian-Xuan Hou  1 , Ai-Guo Xu  2 , Hong-Bo Guo  3 , Xiao-Dan Yu  1 , Rui-Heng Yang  4
Affiliations

Gerhardtia tomentosa and Ossicaulis borealis (Agaricales, Lyophyllaceae)-Two new species from northeast China

Yue Qi et al. Front Microbiol. .

Abstract

Background: Gerhardtia and Ossicaulis are two genera within the family Lyophyllaceae, which show an apparently poor species diversity worldwide. During the field investigation on wild macrofungi, six interesting collections within Gerhardtia and Ossicaulis genera are discovered in the northeastern China.

Methods: To identify whether these collections of Gerhardtia and Ossicaulis are novel species, we performed phylogenetic analyzes using the following DNA regions: the internal transcribed spacer (ITS) region and the large subunit nuclear ribosomal RNA (nrLSU) region. Moreover, a traditional morphological method also be conducted based on both the macro-morphological and micro-morphological features.

Results: The results indicated that these collections tested formed two independent lineages in each genus with a high support. In addition, they can easily be separated from all other taxa of the two genera in morphology. Based on the combination of morphological and molecular data, Gerhardtia tomentosa and Ossicaulis borealis, are confirmed as two new species to science.

Discussions: This study provided a theoretical basis is for the two lyophylloid genera and indicated that the biodiversity resources of northeastern China might be underestimated.

Keywords: Gerhardtia; Lyophyllaceae; Ossicaulis; morphology; phylogeny; taxonomy.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
ML and BI analysis of Lyophyllaceae s.l. based on ITS and nrLSU sequences. PP ≥0.95 (left) and BS ≥60 (right) are indicated on branches. Entoloma sericeonitidum and Nolanea sericea are rooted as the out-groups. Newly generated sequences for Gerhardtia tomentosa and Ossicaulis borealis are highlighted in boxes. GenBank accession numbers are provided after the species name.
Figure 2
Figure 2
ML and BI analysis of Gerhardtia based on ITS and nrLSU sequences. PP ≥0.95 (left) and BS ≥60 (right) are indicated on branches. Calocybella pudica is rooted as the out-group. Newly generated sequences for Gerhardtia tomentosa are highlighted in boxes. GenBank accession numbers are provided after the species name.
Figure 3
Figure 3
ML and BI analysis of Ossicaulis based on ITS and nrLSU sequences. PP ≥0.95 (left) and BS ≥60 (right) are indicated on branches. Hypsizygus ulmarius is rooted as the out-group. Newly generated sequences for Ossicaulis borealis are highlighted in boxes. GenBank accession numbers are provided after the species name.
Figure 4
Figure 4
Gerhardtia tomentosa (Holotype, SYAU-FUNGI-074). (A) macroscopic habitat (B) surface of basidiospores. Scale bars: 1 cm (A); 3 μm (B).
Figure 5
Figure 5
Line drawings of Gerhardtia tomentosa (Holotype, SYAU-FUNGI-074). (a) Basidiospores, (b) basidia and basidioles, (c) Pileipellis, and (d) hyphae from trama.
Figure 6
Figure 6
Ossicaulis borealis (Holotype, SYAU-FUNGI-076). (A) Macroscopic habitat and (B,C,D) surface of basidiospores. Scale bars: 1 cm (A); 2 μm (B).
Figure 7
Figure 7
Line drawings of Ossicaulis borealis (Holotype, SYAU-FUNGI-076). (a) Basidiospores, (b) basidia and basidioles, (c) Pileipellis, and (d) hyphae from trama.
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