Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar 28:8:139.
doi: 10.12688/wellcomeopenres.19226.1. eCollection 2023.

Validation of proteins associated with pathological damage in human tuberculosis granulomas: study protocol

Thabo Mpotje  1   2 , Jessica More  1   2 , Kerishka Rajkumar-Bhugeloo  1   2 , Denelle Moodley  2 , Mohlopheni J Marakalala  1   2   3
Affiliations

Validation of proteins associated with pathological damage in human tuberculosis granulomas: study protocol

Thabo Mpotje et al. Wellcome Open Res. .

Abstract

The presence of the Tuberculosis (TB) disease-causing pathogen, Mycobacterium Tuberculosis (Mtb), induces the development of a pathological feature termed granuloma, which the host uses to contain the bacteria. However, the granuloma may dissociate resulting in detrimental caseation of the lung. The disease contributes to a growing global burden of lung function challenges, warranting for more understanding of the TB-induced immunopathology. The current study aims to explore in detail host factors that drive pathological features of TB contributing to extensive lung tissue destruction. Lung tissue sections obtained from patients undergoing surgical resection will be processed and analyzed using histopathological assays including Immunohistochemistry, Immunofluorescence, Hematoxylin and Eosin staining and Laser Capture Microdissection. The findings will provide key host factors that associate with exacerbated lung immunopathology during TB.

Keywords: Host-Directed Therapies; Inflammation; Lung pathology; Mycobacterium tuberculosis; TB Granulomas.

PubMed Disclaimer

Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.
Representative images of solid ( A), caseous ( B) and cavitary tuberculosis granulomas. Adapted from Marakalala et al., 2016.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Amaral AFS, Coton S, Kato B, et al. : Tuberculosis Associates with Both Airflow Obstruction and Low Lung Function: BOLD Results. Eur Respir J. 2015;46(4):1104–12. 10.1183/13993003.02325-2014 - DOI - PMC - PubMed
    1. Byrne AL, Marais BJ, Mitnick CD, et al. : Tuberculosis and Chronic Respiratory Disease: A Systematic Review. Int J Infect Dis. 2015;32:138–46. 10.1016/j.ijid.2014.12.016 - DOI - PubMed
    1. Datta M, Via LE, Kamoun WS, et al. : Anti-Vascular Endothelial Growth Factor Treatment Normalizes Tuberculosis Granuloma Vasculature and Improves Small Molecule Delivery. Proc Natl Acad Sci U S A. 2015;112(6):1827–32. 10.1073/pnas.1424563112 - DOI - PMC - PubMed
    1. Guler R, Ozturk M, Sabeel S, et al. : Targeting Molecular Inflammatory Pathways in Granuloma as Host-Directed Therapies for Tuberculosis. Front Immunol. 2021;12:733853. 10.3389/fimmu.2021.733853 - DOI - PMC - PubMed
    1. Hnizdo E, Murray J: Risk of Pulmonary Tuberculosis Relative to Silicosis and Exposure to Silica Dust in South African Gold Miners. Occup Environ Med. 1998;55(7):496–502. 10.1136/oem.55.7.496 - DOI - PMC - PubMed

LinkOut - more resources