The association between TIPARP gene polymorphisms rs2665390 and ovarian cancer susceptibility

Abstract

Ovarian cancer is taken as the most typical malignancy among women and the ninth most typical cancer in Iran. Predictive tools are of great importance as ovarian cancer is usually detected in patients at later stages of the disease. In other countries, the TIPARP gene rs2665390 has been reported to be pertinent to ovarian cancer as a risk factor. This study aims to examine if this polymorphism pertains to the risk of ovarian cancer to diagnose suitable biomarkers in the Iranian population. Method: In the present case-control piliot study, peripheral blood samples were gathered from 60 control subjects and 60 patients with ovarian cancer. The gene was determined by Tetra ARMS PCR after DNA extraction. Tetra ARMS PCR is a flexible, rapid, and cost-effective method to detect allele-specific DNA polymorphisms. The data were analyzed by chi-square test. Results: The results indicated that there was a significant association between the T/T and C/C genotypes distribution and C and T allele in ovarian cancer for rs2665390 polymorphism in the two populations. In addition, significant correlations were observed in patients with the (T/T) genotype (p = 0.0048) as frequencies of ovarian cancer decreased. Discussion & Conclusions: Based on the results, rs2665390 polymorphism of TiPARP gene might be pertained to the susceptibility of ovarian cancer in the Iranian pilot population, which can be used as a suitable biomarker for the population and help physicians with their predictions. However, more studies need to be conducted in this area to broaden our horizons on this issue.

Keywords: Ovarian cancer; Rs2665390; Single nucleotide polymorphism; TIPARP gene; Tetra-ARMs PCR.

Fig. 1

Tetra ARMS PCR Products of Different Alleles of TIPARP gene rs2665390 from left to right: lane 1: heterozygote CT genotype; lane 2: homozygote CC; lane 3: homozygote TT genotype; lane 4: 50 bp ladder. Tetra ARMS-PCR was used to specify varying genotypes of the TIPARP gene rs2665390 polymorphism.