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. 2023 Apr 6;8(3):335-339.
doi: 10.1016/j.ncrna.2023.03.006. eCollection 2023 Sep.

Abnormal expression of MAPK14-related lncRNAs in the peripheral blood of patients with multiple sclerosis

Soudeh Ghafouri-Fard  1 , Mahdi Gholipour  2 , Solat Eslami  3   4 , Bashdar Mahmud Hussen  5 , Mohammad Taheri  6   7 , Mohammad Samadian  8 , Mir Davood Omrani  9
Affiliations

Abnormal expression of MAPK14-related lncRNAs in the peripheral blood of patients with multiple sclerosis

Soudeh Ghafouri-Fard et al. Noncoding RNA Res. .

Abstract

Introduction: Contribution of MAPK14 in the pathogenesis of multiple sclerosis (MS) has been proposed by several studies. Long non-coding RNA (lncRNA) have been suggested to be functionally linked with Mitogen-activated protein kinase 14 (MAPK14).

Methods: Expression levels of MAPK14 and its associated lncRNAs were measured in the circulation of MS patients compared with control subjects.

Results: Expression levels of NORAD and RAD51-AS1 were higher in total patients compared with controls (Expression ratio (95% CI) = 1.4 (1.04-1.89), P value = 0.015 and Expression ratio (95% CI) = 1.91 (1.43-2.6), P value = 0.0001, respectively). Conversely, ZNRD1ASP was under-expressed in cases compared with controls (Expression ratio (95% CI) = 0.61 (0.41-0.8), P value = 0.0005). In spite of the observed abnormal expression levels of these lncRNAs in the circulation of MS patients, their expressions were not correlated with Expanded Disability Status Scale (EDSS) score, disease duration or age at disease onset.

Conclusion: To sum up, the current investigation shows dysregulation of MAPK14-related lncRNAs in MS patients.

Keywords: HCG11; Multiple sclerosis; NORAD; RAD51-AS1; ZNRD1ASP; lncRNA.

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Conflict of interest statement

The authors declare they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Relative expression levels of MAPK14 gene and four related lncRNAs in MS patients and healthy controls as described by –delta Ct values (Ct Housekeeping gene- Ct Target gene). Mann-Whitney U test was used to identify differentially expressed genes between two groups (*P value < 0.05 and ***P value < 0.001).
Fig. 2
Fig. 2
Relative expression levels of MAPK14 gene and four related lncRNAs in Multiple Sclerosis (MS) patients (male and female) versus control subgroups (male and female) as described by –delta Ct values. Two-way ANOVA and Tukey post hoc tests were used to analyze the effects of main factors (disease and gender) and the interaction on gene expression levels in subgroups (*P value < 0.05, **P value < 0.01 and ***P value < 0.001).
Fig. 3
Fig. 3
Receiver operating characteristic (ROC) curves of RAD51-AS1, ZNRD1ASP and NORAD lncRNAs transcript levels in MS (AUC shows area under curves).
See this image and copyright information in PMC

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