Streptomyces phytophilus sp. nov., an endophytic actinobacterium with biosynthesis potential as an antibiotic producer

Abstract

An endophytic actinobacterium, strain PIP175^T, was isolated from the root sample of a native apricot tree (Pittosporum angustifolium) growing on the Bedford Park campus of Flinders University, Adelaide, South Australia. This strain is a Gram stain-positive, aerobic actinobacterium with well-developed substrate mycelia. Aerial mycelia rarely produce spores and the spore chain is spiral. Strain PIP175^T showed the highest 16S rRNA gene sequence similarity to Streptomyces aculeolatus DSM 41644^T (99.4 %). Other closely related phylogenetic representatives include Streptomyces synnematoformans DSM 41902^T (98.3 %), Streptomyces albospinus NBRC 13846^T (97.6 %), Streptomyces cacaoi subsp. cacaoi NRRL B-1220^T (97.5 %) and Streptomyces ruber NBRC 14600^T (97.4 %). The major cellular fatty acid of this strain was iso-C_{16 : 0} and the major menaquinone was MK-9(H₆). The whole-cell sugar contained galactose, glucose and mannose. Chemotaxonomic data confirmed that strain PIP175^T belonged to the genus Streptomyces. Digital DNA-DNA hybridization, average nucleotide identity based on blast and OrthoANIu results between strain PIP175^T and S. aculeolatus DSM 41644^T were 60.0, 94.1 and 94.9 %, respectively. Genotypic and phenotypic data and genome analysis results allowed the differentiation of strain PIP175^T from its closest species with validly published names. Strain PIP175^T showed good activity against methicillin-resistant Staphylococcus aureus 03120385. Genome mining of strain PIP175^T revealed biosynthetic genes encoding proteins relating to antibiotic production, plant growth promotion and biodegradation enzymes. The name proposed for the new species is Streptomyces phytophilus sp. nov. The type strain is PIP175^T (=DSM 103379^T=TBRC 6026^T).

Keywords: Streptomyces phytophilus sp. nov.; actinobacterium; endophyte; genome mining.