Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar 31;9(4):e14938.
doi: 10.1016/j.heliyon.2023.e14938. eCollection 2023 Apr.

Comparison of alternative splicing (AS) events in adipose tissue of polled dorset versus small tail han sheep

Xiangyang Miao  1 , Qingmiao Luo  1 , Huijing Zhao  1 , Xiaoyu Qin  1
Affiliations

Comparison of alternative splicing (AS) events in adipose tissue of polled dorset versus small tail han sheep

Xiangyang Miao et al. Heliyon. .

Abstract

Background: During the alternative splicing (AS), the exons of primary transcripts are spliced in various arrangements, resulting in structurally and functionally distinct mRNAs and proteins. This study aimed to examine genes with AS events from Small Tail Han sheep and Dorset sheep to explore the mechanism of adipose developments.

Methods: This study identified the genes with AS events in adipose tissues of two different sheep with next-generation sequencing. In this paper, genes with significantly different AS events were performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses.

Results: 364 genes with 411 A S events showed significant differences in adipose tissues between the two breeds; 108 genes with 120 A S events were extremely significant differences between the two breeds. We identified several novel genes that are related with adipose growth and development. The results of KEGG and GO analysis indicated that oocyte meiosis, mitogen-activated protein kinase (Wnt), mitogen-activated protein kinase (MAPK) signaling pathway, etc. Were closely related to the adipose tissue developments.

Conclusions: This paper revealed that the genes with AS events are important for adipose tissues in sheep, exploring the mechanisms of AS events associated with adipose tissue developments in sheep of different breeds.

Keywords: Adipose; Alternative splicing; Sheep; Transcriptome; mRNA.

PubMed Disclaimer

Conflict of interest statement

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Figures

Fig. 1
Fig. 1
Alternative splicing events identified between two sheep breeds A: all number of alternative splicing events in this study. B: identified alternative splicing events with significant differences. C: identified alternative splicing events with extremely significant differences.
Fig. 2
Fig. 2
KEGG enrichment analysis of all the 364 genes with differential AS events identified in this study. The significance level of enrichment was set at p-value >0.5.
Fig. 3
Fig. 3
108 genes with extremely significant difference of the identified alternative splicing events. The significance level of enrichment was set at p-value >0.01.
Fig. 4
Fig. 4
KEGG analyses of 108 genes with extremely significant difference AS events identified in this study.
Fig. 5
Fig. 5
GO enrichment analysis of 364 genes with differential AS events identified in this study. The figure is composed of three parts: “biological processes (BP)”, “molecular functions (MF)” and “cellular components (CC)”. Hypergeometric statistical test methods were used for analysis, and the significance level of enrichment was set at p-value <1e−4. Black solid lines symbolize the connections between enriched terms. These boxes contain GO functional positioning that is equivalent to the significant GO terms.
Fig. 6
Fig. 6
GO enrichment analysis of 108 genes with extremely differential AS events identified in this study. The figure is composed of three parts: “biological processes (BP)”, “molecular functions (MF)” and “cellular components (CC)”. Hypergeometric statistical test methods were used for analysis, and the significance level of enrichment was set at p value < 0.1. Black solid lines symbolize the connections between enriched terms. The boxes contain GO functional positioning that is equivalent to the significant GO terms.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Atti N., Bocquier F., Khaldi G. Performance of the fat-tailed Barbarine sheep in its environment: adaptive capacity to alternation of underfeeding and re-feeding periods. Anim. Res. 2004;53:165–176.
    1. Miao X.Y., Luo Q.M., Qin X.Y. Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Mol. Cell. Endocrinol. 2015;40:232–242. - PubMed
    1. Miao X.Y., Luo Q.M., Zhao H.J., Qin X.Y. Ovarian proteomic study reveals the possible molecular mechanism for hyperprolificacy of Small Tail Han sheep. Sci. Rep. 2016;6 - PMC - PubMed
    1. Miao X.Y., Luo Q.M., Qin X.Y., Guo Y.T. Genome-wide analysis of microRNAs identifies the lipid metabolism pathway to be a defining factor in adipose tissue from different sheep. Sci. Rep. 2015;5 - PMC - PubMed
    1. Miao X.Y., Luo Q.M., Qin X.Y., Guo Y.Y., Zhao H.J. Genome-wide mRNA-seq profiling reveals predominant down-regulation of lipid metabolic processes in adipose tissues of Small Tail Han than Dorset sheep. Biochem. Biophys. Res. Commun. 2015;467:413–420. - PubMed