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. 1986 May 12;200(2):287-90.
doi: 10.1016/0014-5793(86)81154-1.

Highly selective affinity labelling of RNA polymerase B (II) from wheat germ

M A Grachev, G R Hartmann, T G Maximova, A A Mustaev, A R Schäffner, H Sieber, E F Zaychikov
Free article

Highly selective affinity labelling of RNA polymerase B (II) from wheat germ

M A Grachev et al. FEBS Lett. .
Free article

Abstract

DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(beta-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [alpha-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with Mr 140 000 by covalently linked ApU. Labelling was inhibited by 1 microgram/ml alpha-amanitin.

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