doi: 10.1016/0014-5793(86)81154-1.
Highly selective affinity labelling of RNA polymerase B (II) from wheat germ
- PMID: 3709795
- DOI: 10.1016/0014-5793(86)81154-1
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Highly selective affinity labelling of RNA polymerase B (II) from wheat germ
FEBS Lett.
.
Free article
Abstract
DNA-dependent RNA polymerase B (II) from wheat germ was modified by incubation with 4-[N-(beta-hydroxyethyl)-N-methyl]benzaldehyde esters of AMP, ADP or ATP, followed by reduction with NaBH4. Reaction of the modified enzyme with [alpha-32P]UTP in the presence of various DNA templates led to a highly selective affinity labelling of the subunit with Mr 140 000 by covalently linked ApU. Labelling was inhibited by 1 microgram/ml alpha-amanitin.
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