Effect of cystic fibrosis and non-cystic fibrosis plasma on 45Ca and 35SO4 uptake and retention by the small intestine at the cellular level in the guinea pig

Abstract

A highly significant increase in net fluxes across the guinea-pig small intestine for both 45Ca2+ and 35SO4(2-), in the presence of cystic fibrosis plasma compared with non-cystic fibrosis plasma, was observed (p less than 0.001). In the presence of cystic fibrosis plasma, the nuclear, mitochondrial, microsomal and soluble fractions retained greater activity when compared with non-cystic fibrosis plasma. Of all the fractions, the nuclear fraction retained the highest activity both for 45Ca2+ and 35SO4(2-). These findings indicate that cystic fibrosis plasma increases the net fluxes and raises the retention of 45Ca2+ and 35SO4(2-) in guinea-pig small intestine at the cellular level.