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. 2023 Apr 27:151:e76.
doi: 10.1017/S0950268823000560.

Effectiveness of near-UVA in SARS-CoV-2 inactivation

Eleonora Frilli  1 , Davide Amodeo  2 , Gabriele Cevenini  3 , Nicola Nante  1   2 , Gabriele Messina  1   2
Affiliations

Effectiveness of near-UVA in SARS-CoV-2 inactivation

Eleonora Frilli et al. Epidemiol Infect. .

Abstract

This experimental study aimed to determine the activity of a near-UVA (405 nm) LED ceiling system against the SARS-CoV-2 virus. The ceiling system comprised 17 near-UVA LED lights with a radiant power of 1.1 W/each centred at 405 nm wavelength. A 96-multiwell plate, fixed to a wooden base, was inoculated with suspensions of VERO E6 cell cultures infected with SARS-CoV-2 virus and irradiated at a distance of 40 cm with a dose of 20.2 J/cm2 for 120 min. The collected suspensions were transferred to VERO cell culture plates and incubated for 3 days. The maximum measurable log reduction obtained, starting from a concentration of 107.2 TCID50/mL, was 3.0 log10 and indicated inhibition of SARS-CoV-2 replication by the near-UVA LED ceiling system. Near-UVA light at a 405-nm wavelength is emerging as a potential alternative treatment for localised infections and environmental decontamination because it is far less harmful to living organisms' cells than UV-C irradiation.

Keywords: COVID-19; Cell Colture; Disinfection; Hygiene; Prevention; SARS-CoV-2; UVC; near-UVA; virus infection.

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Conflict of interest statement

Ugolini srl partially financed the University of Siena for research and development (R&D) activities related to devices adopting the described technology where G.M. was the principal investigator. R&D led to a patent application owned by Ugolini srl where G.C. and G.M. were co-inventors G.C. and G.M. are co-founders of the company egoHEALTH which was financed by Ugolini srl to cover minor parts of the investigation, included fees for patent activity issues. G.C. and G.M. did not receive any personal funds for the research. Ugolini srl had no role in any part of the manuscript.

Figures

Figure 1.
Figure 1.
Spectral behaviour as a function of wavelength (nm) of the near-UVA LEDs Luminus SST-10-UV (blue line).
Figure 2.
Figure 2.
(a) Example of the 24 multiwell plate and selected wells used for the test (green spots C2 on the left, C3 in the centre, and C4 on the right). (b) Photometric measurements were carried out with the cosine corrector probe of the Avantes spectrophotometer to detect the irradiance values present in each of the three wells of the plate.
Figure 3.
Figure 3.
Experiment setting designed for the tests with the 405 nm LED ceiling system.
See this image and copyright information in PMC

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