Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug;47(8):732-742.
doi: 10.1038/s41366-023-01317-1. Epub 2023 Apr 26.

Visceral mesenchymal stem cells from type 2 diabetes donors activate triglycerides synthesis in healthy adipocytes via metabolites exchange and cytokines secretion

Iurii Stafeev  1 , Svetlana Michurina  2   3 , Margarita Agareva  2   4 , Ekaterina Zubkova  2 , Igor Sklyanik  5 , Ekaterina Shestakova  5 , Alina Gavrilova  5 , Maria Sineokaya  5 , Elizaveta Ratner  2 , Mikhail Menshikov  2 , Yelena Parfyonova  2   3 , Marina Shestakova  5
Affiliations

Visceral mesenchymal stem cells from type 2 diabetes donors activate triglycerides synthesis in healthy adipocytes via metabolites exchange and cytokines secretion

Iurii Stafeev et al. Int J Obes (Lond). 2023 Aug.

Abstract

Background: In recent years, there has been an increase in the prevalence of obesity and type 2 diabetes mellitus (T2DM). Development of visceral instead of subcutaneous adipose tissue is pathogenic and increases the risk of metabolic abnormalities. We hypothesize that visceral adipocytes and stromal cells are able to deteriorate other fat depots metabolism via secretory mechanisms.

Methods: We study the regulatory role of visceral adipose-derived stem cells (vADSC) from donors with obesity and T2DM or normal glucose tolerance (NGT) on healthy subcutaneous ADSC (sADSC) in the Transwell system. Lipid droplets formation during adipogenesis was assessed by confocal microscopy. Cell metabolism was evaluated by 14C-glucose incorporation analysis and western blotting. vADSC secretome was assessed by Milliplex assay.

Results: We showed that both NGT and T2DM vADSC had mesenchymal phenotype, but expression of CD29 was enhanced, whereas expressions of CD90, CD140b and IGF1R were suppressed in both NGT and T2DM vADSC. Co-differentiation with T2DM vADSC increased lipid droplet size and stimulated accumulation of fatty acids in adipocytes from healthy sADSC. In mature adipocytes T2DM vADSC stimulated triglyceride formation, whereas NGT vADSC activated oxidative metabolism. Secretome of NGT vADSC was pro-inflammatory and pro-angiogenic in comparison with T2DM vADSC.

Conclusions: The present study has demonstrated the critical role of secretory interactions between visceral and subcutaneous fat depots both in the level of progenitor and mature cells. Mechanisms of these interactions are related to direct exchange of metabolites and cytokines secretion.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Ladabaum U, Mannalithara A, Myer PA, Singh G. Obesity, abdominal obesity, physical activity, and caloric intake in U.S. adults: 1988–2010. Am J Med. 2014;127:717–27.e12. - PubMed - PMC
    1. Hall KD, Ayuketah A, Brychta R, Cai H, Cassimatis T, Chen KY, et al. Ultra-processed diets cause excess calorie intake and weight gain: An inpatient randomized controlled trial of ad libitum food intake. Cell Metab. 2019;30:67–77.e3. - PubMed - PMC
    1. Liu Y, Wang X, Zhang Q, Meng G, Liu L, Wu H, et al. Relationship between dietary patterns and carotid atherosclerosis among people aged 50 years or older: A population-based study in China. Front Nutr. 2021;8,723726.
    1. Yang T, Zhao B, Pei D. Evaluation of the association between obesity markers and type 2 diabetes: a cohort study based on a physical examination population, J.Diabetes.Res. 2021;6503339.
    1. American Diabetes Association Professional Practice Committee, Draznin B, Aroda VR, Bakris G, Benson G, Brown FM, et al. Obesity and weight management for the prevention and treatment of type 2 diabetes: standards of medical care in diabetes-2022. Diabetes Care. 2022;45:S113–S124.

Publication types

LinkOut - more resources