Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr 25;13(1):6773.
doi: 10.1038/s41598-023-33992-5.

Characterization and investigation of biological properties of silver nanoparticle-doped hydroxyapatite-based surfaces on zirconium

Tuba Yildiz  1 , Salih Durdu  2   3   4 , Kadriye Ozcan  5 , Metin Usta  6   7
Affiliations

Characterization and investigation of biological properties of silver nanoparticle-doped hydroxyapatite-based surfaces on zirconium

Tuba Yildiz et al. Sci Rep. .

Abstract

The infections leading to failed implants can be controlled mainly by metal and metal oxide-based nanoparticles. In this work, the randomly distributed AgNPs-doped onto hydroxyapatite-based surfaces were produced on zirconium by micro arc oxidation (MAO) and electrochemical deposition processes. The surfaces were characterized by XRD, SEM, EDX mapping and EDX area and contact angle goniometer. AgNPs-doped MAO surfaces, which is beneficial for bone tissue growth exhibited hydrophilic behaviors. The bioactivity of the AgNPs-doped MAO surfaces is improved compared to bare Zr substrate under SBF conditions. Importantly, the AgNPs-doped MAO surfaces exhibited antimicrobial activity for E. coli and S. aureus compared to control samples.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Schematic representation of experimental set up: (a) MAO system and (b) ED system.
Figure 2
Figure 2
XRD spectra of the MAO and AgNPs-doped MAO surfaces.
Figure 3
Figure 3
XPS spectra of AgNPs-doped MAO surface produced at 15 min: (a) Survey, (b) Zr3d, (c) Ag3d, (d) Ag3p, (e) Ca2p and (f) O1s.
Figure 4
Figure 4
Surface topography of the MAO surfaces taken at low magnifications: (a) 15 min, (b) 30 min and (c) 45 min.
Figure 5
Figure 5
Surface topography of the MAO and AgNPs-doped MAO surfaces taken at high magnifications: (a) MAO-15, (b) MAO-15-Ag, (c) MAO-30, (d) MAO-30-Ag, (e) MAO-45 and (d) MAO-45-Ag.
Figure 6
Figure 6
EDX-mapping images of AgNPs-doped MAO surfaces.
Figure 7
Figure 7
Contact angle images of the surfaces: (a) bare Zr, (b) MAO-30 and (c) MAO-30-Ag.
Figure 8
Figure 8
Surface topography of the surfaces at post-immersion in SBF for 10 days: (a) bare Zr, (b) MAO-15-Ag, (c) MAO-30-Ag and (d) MAO-45-Ag.
Figure 9
Figure 9
XRD spectra of AgNPs-doped MAO surfaces at post immersion in SBF.
Figure 10
Figure 10
Reduction in microbial colonies after re-culturation in samples with the highest antibacterial activity on MAO-45-Ag: (a) E. coli viability after re-culture on bare Zr surfaces, (b) E. coli viability after re-culture on MAO-45-Ag surfaces, (c) S. aureus viability after re-culture on bare Zr surfaces, (b) S. aureus viability after re-culture on MAO-45-Ag surfaces.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Sanchez AG, Schreiner W, Duffo G, Cere S. Surface characterization of anodized zirconium for biomedical applications. Appl. Surf. Sci. 2011;257:6397–6405. doi: 10.1016/j.apsusc.2011.02.005. - DOI
    1. Hosseini MG, Daneshvari-Esfahlan V, Maleki-Ghaleh H. Effect of water and fluoride content of anodizing electrolyte on morphology and corrosion behavior of ZrO2-nanotubes developed on zirconium implant. J. Mater. Eng. Perform. 2016;25:1129–1135. doi: 10.1007/s11665-016-1904-z. - DOI
    1. Cheng Y, Matykina E, Skeldon P, Thompson G. Characterization of plasma electrolytic oxidation coatings on Zircaloy-4 formed in different electrolytes with AC current regime. Electrochim. Acta. 2011;56:8467–8476. doi: 10.1016/j.electacta.2011.07.034. - DOI
    1. Molaei M, Attarzadeh N, Fattah-alhosseini A. Tailoring the biological response of zirconium implants using zirconia bioceramic coatings: A systematic review. J. Trace Elements Med. Biol. 2021;66:126756. doi: 10.1016/j.jtemb.2021.126756. - DOI - PubMed
    1. Chopra D, Gulati K, Ivanovski S. Micro + nano: Conserving the gold standard microroughness to nanoengineer zirconium dental implants. ACS Biomater. Sci. Eng. 2021;7:3069–3074. doi: 10.1021/acsbiomaterials.1c00356. - DOI - PubMed