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. 2023 Apr 26;23(1):197.
doi: 10.1186/s12887-023-04020-2.

The potential of tRF-21-U0EZY9X1B plasmatic level as a biomarker of children with obstructive sleep apnea-hypopnea syndrome

Yanbo Lu #  1 , Qiang Fu #  2 , Xiaohong Cai  1 , Yijing Shen  3 , Junhua Wu  4 , Haiyan Qiu  5
Affiliations

The potential of tRF-21-U0EZY9X1B plasmatic level as a biomarker of children with obstructive sleep apnea-hypopnea syndrome

Yanbo Lu et al. BMC Pediatr. .

Abstract

Purpose: We investigated changes in plasma transfer RNA related fragments (tRF) in children with obstructive sleep apnea-hypopnea syndrome (OSAHS) and the potential value as a disease marker.

Methods: Firstly, we randomly selected five plasma samples from the case group and the control group for high-throughput RNA sequencing. Secondly, we screened one tRF with different expression between the two groups, amplified it by quantitative reverse transcription-PCR (qRT-PCR) and sequenced the amplified product. After confirming that the qRT-PCR results were consistent with the sequencing results and the sequence of the amplified product contained the original sequence of the tRF, we performed qRT-PCR on all samples. Then we analyzed the diagnostic value of the tRF and its correlation with some clinical data.

Results: A total of 50 OSAHS children and 38 control children were included in this study. There were significant differences in height, serum creatinine (SCR) and total cholesterol (TC) between the two groups. The plasma expression levels of tRF-21-U0EZY9X1B (tRF-21) were significantly different between the two groups. Receiver operating characteristic curve (ROC) showed that it had valuable diagnostic index, with area under the curve (AUC) of 0.773, 86.71% and 63.16% sensitivity and specificity.

Conclusions: The expression levels of tRF-21 in the plasma of OSAHS children decreased significantly which were closely related to hemoglobin, mean corpuscular hemoglobin, triglyceride and creatine kinase-MB, may become novel biomarkers for the diagnosis of pediatric OSAHS.

Keywords: Biomarker; Children; Obstructive sleep apnea-hypopnea syndrome; tRNA-derived fragments.

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Conflict of interest statement

The authors have no relevant financial or non-financial interests to disclose.

Figures

Fig. 1
Fig. 1
Sequence results of qRT-PCR products of tRF-21-U0EZY9X1B
Fig. 2
Fig. 2
Differentially expressed genes for tsRNA levels in plasma of 5 healthy people and 5 children with OSAHS. The values of X and Y axes in the scatter plot are the averaged CPM values of each roup (log2 saled). tsRNAs above the top line (red dots, up-regulation) or below the bottom ine (green dots, down-regulation) indicate more than 1.5 folds change between the two compared groups. ray dots indicate non-differentially expressed tsNAs.
Fig. 3
Fig. 3
The Pie showed the tsRNA subtypes. Different colors represented the numbers of tsRNA subtypes and the number of subtypes was marked next to it
Fig. 4
Fig. 4
Differential expression and diagnostic value of tRF-21-U0EZY9X1B. Student t-test of detecting the expression difference of tRF-21-U0EZY9X1B between the two groups (A) (t = 3.969, 95% CI: -6.194 to -2.059); Receiver operating characteristic curve of tRF-21-U0EZY9X1B (B) (95% CI: 0.666 to 0.881)
Fig. 5
Fig. 5
Scatter plot of correlation between tRF-21-U0EZY9X1B and degree of tonsil enlargement (A), Hb (B), MCH (C), TG (D) and CK-MB (E)
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