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. 2023 Jun;597(12):1623-1637.
doi: 10.1002/1873-3468.14630. Epub 2023 May 8.

The MIR34B/C genomic region contains multiple potential regulators of multiciliogenesis

Amélie Cavard  1 , Elisa Redman  1 , Olivier Mercey  2 , Sophie Abelanet  1 , Magali Plaisant  1 , Marie-Jeanne Arguel  1 , Virginie Magnone  1 , Sandra Ruiz García  1 , Géraldine Rios  1 , Marie Deprez  1 , Kévin Lebrigand  1 , Gilles Ponzio  1 , Ignacio Caballero  3 , Pascal Barbry  1 , Laure-Emmanuelle Zaragosi  1 , Brice Marcet  1
Affiliations
Free article

The MIR34B/C genomic region contains multiple potential regulators of multiciliogenesis

Amélie Cavard et al. FEBS Lett. 2023 Jun.
Free article

Abstract

The MIR449 genomic locus encompasses several regulators of multiciliated cell (MCC) formation (multiciliogenesis). The miR-449 homologs miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN, and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse, or pig multiciliogenesis models. BTG4, LAYN, and HOATZ transcripts were expressed in both precursors and mature MCCs. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.

Keywords: BTG4; HOATZ; LAYN; miR-34b/c; motile cilia; multiciliated cells.

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References

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