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. 2023 Apr 18;12(8):1682.
doi: 10.3390/foods12081682.

Effects of Ficus pandurata Hance var. angustifolia Cheng Flavonoids on Intestinal Barrier and Cognitive Function by Regulating Intestinal Microbiota

Yuting Zhang  1 , Junjie Pan  2 , Yanan Liu  1 , Xin Zhang  1 , Kejun Cheng  1   2
Affiliations

Effects of Ficus pandurata Hance var. angustifolia Cheng Flavonoids on Intestinal Barrier and Cognitive Function by Regulating Intestinal Microbiota

Yuting Zhang et al. Foods. .

Abstract

More and more evidence has supported the interaction between circadian rhythms and intestinal microbes, which provides new insights into how dietary nutrition can improve host health. Our research showed that Ficus pandurata Hance var. angustifolia Cheng flavonoids (FCF) ameliorated the pathological damage of colon and abnormal intestinal microflora structure in mice with circadian clock disorder and improved their exploration and memory behaviors. Mechanism studies have shown that FCF is involved in regulating metabolic pathways and related metabolites, regulating the expression of related tight junction proteins in the colon and the levels of Aβ and inflammatory factors in the hippocampus. Further analysis found that these metabolites showed a certain correlation with intestinal flora and played a certain role in alleviating intestinal physiological damage and cognitive decline.

Keywords: Ficus pandurata Hance var. angustifolia Cheng flavonoids; cognitive function; intestinal barrier; intestinal microbiota.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
(A) Effect of FCF on the body weight of circadian rhythm disorder mice. * p < 0.05, compared with the CD group. The effect of FCF on the total number of arm entries (B) and the correct rate of spontaneous alternation (C) in mice with circadian rhythm disorder. The effect of FCF on the total 30 min exercise distance (D) and the times of entering the central area (E) in mice with circadian rhythm disorder. * p < 0.05, ** p < 0.01, compared with the CD group.
Figure 2
Figure 2
(A) Effect of FCF supplementation on intestinal histomorphology in mice with circadian rhythm disruption (HE, ×400). (B) Immunohistochemistry of occludin and ZO-1 of the three groups (Immunohistochemistry, ×400). Effects of FCF on the expression of tight junction proteins occludin (C) and ZO-1 (D) in the colon of mice with circadian rhythm disorder. ** p < 0.01, *** p < 0.001, compared with the CD group.
Figure 3
Figure 3
(A) Principal component analysis of fecal microbiota of the three groups. (B) The relative abundance of intestinal flora at the phylum in each group (Weeks 0 and 4). The comparison of relative abundance of the intestinal microbiota at genus in the 4th week between CD and CT group (C), CD and FCF group (D).
Figure 4
Figure 4
(A) HMDB super class identification classification and annotation diagram of metabolites. (B) Volcano plots of the different metabolites in the FCF and the CD groups. (C) Heatmap of the different metabolites between the FCF group and the CD group. (D) KEGG enrichment pathways of different metabolites between the CD and CT groups. (E) KEGG enrichment pathways of regulated differential metabolites between the CD group and FCF group.
Figure 5
Figure 5
(A) The correlation between intestinal microbiota at phylum and differential metabolites between the CD group and FCF group * p < 0.05, ** p < 0.01, compared with the CD group. (B) Network diagram of intestinal differential metabolites and microbiota at the genus levels.
Figure 6
Figure 6
(A) Effects of FCF on hippocampal histomorphology in mice with circadian disturbance (HE, ×200 and ×400). Effects of FCF on the contents of proinflammatory factor IL-1β (B), IL-6 (C) and anti-inflammatory IL-10 (D) in the hippocampus of mice with circadian rhythm disturbance. (E) Results of Aβ fluorescence staining in the hippocampus of each group (×400). (F) Effect of FCF on hippocampal Aβ level in mice with circadian disturbance. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the CD group.
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