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. 2023 Apr 21;14(4):950.
doi: 10.3390/genes14040950.

The Glutathione Peroxidase Gene Family in Nitraria sibirica: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions

Ziming Lian  1   2 , Jingbo Zhang  3 , Zhaodong Hao  1 , Liming Zhu  1 , Yuxin Liu  1   2 , Hao Fang  2 , Ye Lu  1 , Xinle Li  3 , Jisen Shi  1 , Jinhui Chen  1 , Tielong Cheng  2
Affiliations

The Glutathione Peroxidase Gene Family in Nitraria sibirica: Genome-Wide Identification, Classification, and Gene Expression Analysis under Stress Conditions

Ziming Lian et al. Genes (Basel). .

Abstract

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain H2O2 homeostasis and normalize plant reaction to abiotic stress conditions. However, the genome-wide identification of the GPX gene family and its responses to environmental stresses, especially salt stress, in Nitraria sibirica, which is a shrub that can survive in saline environments, has not yet been reported. Here, we first report the genome-wide analysis of the GPX gene family in N. sibirica, leading to a total of seven NsGPX genes that are distributed on six of the twelve chromosomes. Phylogenetic analysis showed that NsGPX genes were grouped into four major groups (Group I-IV). Three types of cis-acting elements were identified in the NsGPX promoters, mainly related to hormones and stress response. The quantitative real-time PCR (qRT-PCR) analysis indicated that NsGPX1 and NsGPX3 were significantly up-regulated in stem and leaf, while NsGPX7 transcriptionally in root in response to salt stress. The current study identified a total seven NsGPX genes in N. sibirica via genome-wide analysis, and discovered that NsGPXs may play an important role in response to salt stress. Taken together, our findings provide a basis for further functional studies of NsGPX genes, especially in regarding to the resistance to salt stress of this halophyte plant N. sibirica, eventually aid in the discovery of new methods to restore overtly saline soil.

Keywords: Nitraria sibirica; gene expression; genomics; glutathione peroxidase; salt stress.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
A neighbor-joining Phylogenetic analyses of 25 plant glutathione peroxidase (GPX) proteins. The plant GPXs include 8 AtGPXs (red), 5 OsGPXs (yellow), 6VvGPXs (green) and 7NsGPXs (blue), symbolized by unique colors.
Figure 2
Figure 2
Chromosome location of NsGPX genes. Each chromosome gene names of each GPX genes on the right side coincide to their approximate locations.
Figure 3
Figure 3
Synteny analyses of GPX genes in N. sibirica, A. thaliana, and V. vinifera chromosomes. Background gray lines represent the collinear blocks within the genomes of N. sibirica and other plants, meanwhile the red lines highlight the syntenic GPX gene pairs.
Figure 4
Figure 4
Domain distribution and conservative motif distribution of NsGPX genes. The names of NsGPXs conserved motifs configurations are given. Specific color blocks represent unique motifs. The CDS regions of NsGPXs are indicated by light blue, whilist the NsGPXs UTR regions are represented by purple. The numbers in the right of color blocks are phase number of CDS.
Figure 5
Figure 5
Evaluation of cis-regulatory elements in the NsGPXs promoters’ regions that are linked with assorted hormone- and stress-responsive elements. Specific color blocks represent unique identified cis-elements.
Figure 6
Figure 6
Expression profiles of NsGPXs under abiotic stress in root (a), stem (b), leave (c).
See this image and copyright information in PMC

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