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Review
. 2023 Apr 8;24(8):6947.
doi: 10.3390/ijms24086947.

Vibrational Spectroscopy as a Tool for Bioanalytical and Biomonitoring Studies

Sergey K Pirutin  1   2   3 , Shunchao Jia  1 , Alexander I Yusipovich  2 , Mikhail A Shank  1   2 , Evgeniia Yu Parshina  2 , Andrey B Rubin  1   2
Affiliations
Review

Vibrational Spectroscopy as a Tool for Bioanalytical and Biomonitoring Studies

Sergey K Pirutin et al. Int J Mol Sci. .

Abstract

The review briefly describes various types of infrared (IR) and Raman spectroscopy methods. At the beginning of the review, the basic concepts of biological methods of environmental monitoring, namely bioanalytical and biomonitoring methods, are briefly considered. The main part of the review describes the basic principles and concepts of vibration spectroscopy and microspectrophotometry, in particular IR spectroscopy, mid- and near-IR spectroscopy, IR microspectroscopy, Raman spectroscopy, resonance Raman spectroscopy, Surface-enhanced Raman spectroscopy, and Raman microscopy. Examples of the use of various methods of vibration spectroscopy for the study of biological samples, especially in the context of environmental monitoring, are given. Based on the described results, the authors conclude that the near-IR spectroscopy-based methods are the most convenient for environmental studies, and the relevance of the use of IR and Raman spectroscopy in environmental monitoring will increase with time.

Keywords: Raman spectroscopy; biological methods of environmental measurements; infrared spectroscopy; vibrational spectroscopy in environmental studies.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analysis, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Biological methods of environmental studies.
Figure 2
Figure 2
Energy transitions typical for the measurements by infrared and Raman spectroscopy are: (a) IR absorption (energy transitions corresponding to the overtone or fundamental transition are shown for near-IR and mid-IR spectroscopy, respectively); (b) photoluminescence, which often occurs during the study of biological objects by Raman spectroscopy; and (c) various Raman radiation types. Adapted from [29].
Figure 2
Figure 2
Energy transitions typical for the measurements by infrared and Raman spectroscopy are: (a) IR absorption (energy transitions corresponding to the overtone or fundamental transition are shown for near-IR and mid-IR spectroscopy, respectively); (b) photoluminescence, which often occurs during the study of biological objects by Raman spectroscopy; and (c) various Raman radiation types. Adapted from [29].
Figure 3
Figure 3
Schematic diagram of various measurement modes: (a) Fourier transform infrared spectroscopy with attenuated total reflection (ATR-FTIRS); (b) Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS).
Figure 4
Figure 4
Structures and spectra of different carotenoids: (a) Isoprene unit; (b) Structures of different carotenoids. Adapted from [78].
Figure 5
Figure 5
Biofuel sources and production: (a) Esterification reaction; (b) Some types of fatty acids. Adapted from [82].
See this image and copyright information in PMC

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