Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr 20;24(8):7582.
doi: 10.3390/ijms24087582.

Genomic Characterization of Uropathogenic Escherichia coli Isolates from Tertiary Hospitals in Riyadh, Saudi Arabia

Rawan H Aljohani  1   2 , Dalia S ElFeky  3   4 , Abdulrahman A Alswaji  1 , Eisa Alrashidi  1 , Liliane Okdah  1 , Bassam Alalwan  5 , Sameera M Aljohani  1   5   6 , Hanan H Balkhy  7 , Alya Redhwan  8 , Majed F Alghoribi  1   5   6
Affiliations

Genomic Characterization of Uropathogenic Escherichia coli Isolates from Tertiary Hospitals in Riyadh, Saudi Arabia

Rawan H Aljohani et al. Int J Mol Sci. .

Abstract

Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections (UTIs) in hospitalised and non-hospitalised patients. Genomic analysis was used to gain further insight into the molecular characteristics of UPEC isolates from Saudi Arabia. A total of 165 isolates were collected from patients with UTIs between May 2019 and September 2020 from two tertiary hospitals in Riyadh, Saudi Arabia. Identification and antimicrobial susceptibility testing (AST) were performed using the VITEK system. Extended-spectrum β-lactamase (ESBL)-producing isolates (n = 48) were selected for whole genome sequencing (WGS) analysis. In silico analysis revealed that the most common sequence types detected were ST131 (39.6%), ST1193 (12.5%), ST73 (10.4%), and ST10 (8.3%). Our finding showed that blaCTX-M-15 gene was detected in the majority of ESBL isolates (79.2%), followed by blaCTX-M-27 (12.5%) and blaCTX-M-8 (2.1%). ST131 carried blaCTX-M-15 or blaCTX-M-27, and all ST73 and ST1193 carried blaCTX-M-15. The relatively high proportion of ST1193 in this study was notable as a newly emerged lineage in the region, which warrants further monitoring.

Keywords: Saudi Arabia; antimicrobial resistance; extended-spectrum β-lactamases; molecular typing; urinary tract infection; uropathogenic E. coli; whole-genome sequence.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest, H.H.B. has been part of this work during her previous tenure as Professor of Pediatric infectious disease at KSAU-HU and Chairman of the infectious disease research department at KAIMRC.

Figures

Figure 1
Figure 1
SNP-derived phylogenetic tree of the UPEC ESBL strains in this study, compared with MLST, phylogenetic groups, fimH typing, serotyping, and ESBL resistance genes. * Isolates displaying a single-locus variant (SLV).
See this image and copyright information in PMC

References

    1. Huang L., Huang C., Yan Y., Sun L., Li H. Urinary Tract Infection Etiological Profiles and Antibiotic Resistance Patterns Varied Among Different Age Categories: A Retrospective Study From a Tertiary General Hospital During a 12-Year Period. Front. Microbiol. 2022;12:813145. - PMC - PubMed
    1. John A.S., Mboto C.I., Agbo B. A review on the prevalence and predisposing factors responsible for urinary tract infection among adults. Eur. J. Exp. Biol. 2016;6:7–11.
    1. Terlizzi M.E., Gribaudo G., Maffei M.E. UroPathogenic Escherichia coli (UPEC) Infections: Virulence Factors, Bladder Responses, Antibiotic, and Non-antibiotic Antimicrobial Strategies. Front. Microbiol. 2017;8:1566. - PMC - PubMed
    1. Bunduki G.K., Heinz E., Phiri V.S., Noah P., Feasey N., Musaya J. Virulence factors and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from urinary tract infections: A systematic review and meta-analysis. BMC Infect. Dis. 2021;21:753. doi: 10.1186/s12879-021-06435-7. - DOI - PMC - PubMed
    1. Wirth T., Falush D., Lan R., Colles F., Mensa P., Wieler L.H., Karch H., Reeves P.R., Maiden M.C.J., Ochman H., et al. Sex and virulence in Escherichia coli: An evolutionary perspective. Mol. Microbiol. 2006;60:1136–1151. - PMC - PubMed

MeSH terms