Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr 7;15(4):1172.
doi: 10.3390/pharmaceutics15041172.

Immobilized Keratin HPLC Stationary Phase-A Forgotten Model of Transdermal Absorption: To What Molecular and Biological Properties Is It Relevant?

Anna Weronika Sobańska  1 , Elżbieta Brzezińska  1
Affiliations

Immobilized Keratin HPLC Stationary Phase-A Forgotten Model of Transdermal Absorption: To What Molecular and Biological Properties Is It Relevant?

Anna Weronika Sobańska et al. Pharmaceutics. .

Abstract

Chromatographic retention data collected on immobilized keratin (KER) or immobilized artificial membrane (IAM) stationary phases were used to predict skin permeability coefficient (log Kp) and bioconcentration factor (log BCF) of structurally unrelated compounds. Models of both properties contained, apart from chromatographic descriptors, calculated physico-chemical parameters. The log Kp model, containing keratin-based retention factor, has slightly better statistical parameters and is in a better agreement with experimental log Kp data than the model derived from IAM chromatography; both models are applicable primarily to non-ionized compounds.Based on the multiple linear regression (MLR) analyses conducted in this study, it was concluded that immobilized keratin chromatographic support is a moderately useful tool for skin permeability assessment.However, chromatography on immobilized keratin may also be of use for a different purpose-in studies of compounds' bioconcentration in aquatic organisms.

Keywords: bioconcentration factor; biomimetic chromatography; immobilized artificial membrane chromatography; immobilized keratin stationary phase; skin permeability.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Equation (6)—predicted and experimental log Kp vs. reference values.
Figure 2
Figure 2
Equation (7)—predicted and experimental log Kp vs. reference values.
Figure 3
Figure 3
Equation (10)—predicted and experimental log BCF vs. reference values.
Figure 4
Figure 4
Equation (11)—predicted and experimental log BCF vs. reference values.
See this image and copyright information in PMC

References

    1. Todo H. Transdermal Permeation of Drugs in Various Animal Species. Pharmaceutics. 2017;9:33. doi: 10.3390/pharmaceutics9030033. - DOI - PMC - PubMed
    1. Weisbrod A.V., Burkhard L.P., Arnot J., Mekenyan O., Howard P.H., Russom C., Boethling R., Sakuratani Y., Traas T., Bridges T., et al. Workgroup Report: Review of Fish Bioaccumulation Databases Used to Identify Persistent, Bioaccumulative, Toxic Substances. Environ. Health Perspect. 2007;115:255–261. doi: 10.1289/ehp.9424. - DOI - PMC - PubMed
    1. Arnot J.A., Gobas F.A. A Review of Bioconcentration Factor (BCF) and Bioaccumulation Factor (BAF) Assessments for Organic Chemicals in Aquatic Organisms. Environ. Rev. 2006;14:257–297. doi: 10.1139/a06-005. - DOI
    1. Arnot J.A., Gobas F.A.P.C. A Generic QSAR for Assessing the Bioaccumulation Potential of Organic Chemicals in Aquatic Food Webs. QSAR Comb. Sci. 2003;22:337–345. doi: 10.1002/qsar.200390023. - DOI
    1. Grisoni F., Consonni V., Villa S., Vighi M., Todeschini R. QSAR Models for Bioconcentration: Is the Increase in the Complexity Justified by More Accurate Predictions? Chemosphere. 2015;127:171–179. doi: 10.1016/j.chemosphere.2015.01.047. - DOI - PubMed

LinkOut - more resources