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. 2023 Mar 25;11(4):304.
doi: 10.3390/toxics11040304.

Adaptive Mechanisms of Shewanella xiamenensis DCB 2-1 Metallophilicity

Marina Abuladze  1 , Nino Asatiani  1 , Tamar Kartvelishvili  1 , Danil Krivonos  2   3 , Nadezhda Popova  4 , Alexey Safonov  4 , Nelly Sapojnikova  1 , Nikita Yushin  5 , Inga Zinicovscaia  5   6   7
Affiliations

Adaptive Mechanisms of Shewanella xiamenensis DCB 2-1 Metallophilicity

Marina Abuladze et al. Toxics. .

Abstract

The dose-dependent effects of single metals (Zn, Ni, and Cu) and their combinations at steady time-actions on the cell viability of the bacteria Shewanella xiamenensis DCB 2-1, isolated from a radionuclide-contaminated area, have been estimated. The accumulation of metals by Shewanella xiamenensis DCB 2-1 in single and multi-metal systems was assessed using the inductively coupled plasma atomic emission spectroscopy. To estimate the response of the bacteria's antioxidant defense system, doses of 20 and 50 mg/L of single studied metals and 20 mg/L of each metal in their combinations (non-toxic doses, determined by the colony-forming viability assay) were used. Emphasis was given to catalase and superoxide dismutase since they form the primary line of defense against heavy metal action and their regulatory circuit of activity is crucial. The effect of metal ions on total thiol content, an indicator of cellular redox homeostasis, in bacterial cells was evaluated. Genome sequencing of Shewanella xiamenensis DCB 2-1 reveals genes responsible for heavy metal tolerance and detoxification, thereby improving understanding of the potential of the bacterial strain for bioremediation.

Keywords: Shewanella xiamenensis; antioxidants; genome sequencing; heavy metals; metal toxicity.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The dose-dependent impact of zinc, copper, and nickel on the S. xiamenensis DCB2-1 cell population is presented as CFU/mL. The data presented are the mean values ± SD from three separate sets of experiments.
Figure 2
Figure 2
The dose-dependent impact of zinc, copper, and nickel combinations on S. xiamenensis DCB2-1 cell population is presented as log (CFU/mL).
Figure 3
Figure 3
The number of genes associated with metal resistance in S. xiamenensis DCB2-1.
Figure 4
Figure 4
Catalase activity in the cell lysate of S. xiamenensis DCB2-1 under zinc, copper, and nickel single (A) and combination actions (B). Metal concentrations in single systems are 20 and 50 mg/L; metal concentrations in complex systems are 20 mg/L. The data presented are the mean values ± SD from three separate sets of experiments.
Figure 5
Figure 5
SOD activity in the cell lysate of S. xiamenensis DCB2-1 under zinc, copper, and nickel single (A,B) and combination actions (C). Metal concentrations in single systems are 20 and 50 mg/L; metal concentrations in complex systems are 20 mg/L. C—Control sample.
Figure 6
Figure 6
Total thiol content in the cell lysate of S. xiamenensis DCB2-1 under zinc, copper, and nickel single (A) and combination action (B). Metal concentrations in single systems are 20 and 50 mg/L; metal concentrations in complex systems are 20 mg/L. The data presented are the mean values ± SD from three separate sets of experiments.
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