NosZ-II-type N2O-reducing bacteria play dominant roles in determining the release potential of N2O from sediments in the Pearl River Estuary, China

Abstract

The microbial reduction of N₂O serves as a "gatekeeper" for N₂O emissions, determining the flux of N₂O release into the atmosphere. Estuaries are active regions for N₂O emissions, but the microbial functions of N₂O-reducing bacteria in estuarine ecosystems are not well understood. In this study, the ¹⁵N isotope tracer method, qPCR, and high-throughput sequencing were used to analyze N₂O production, reduction, and emission processes in surface sediments of the Pearl River Estuary. The ¹⁵N isotope tracer experiment showed that the N₂O production rates declined and the N₂O reduction potential (R_r, the ratio of N₂O reduction rates to N₂O production rates) increased from upstream to downstream of the Pearl River Estuary, leading to a corresponding decrease of the N₂O emission rates from upstream to downstream. The gene abundance ratio of nosZ/nir gradually increased from upstream to downstream and was negatively correlated with the water N₂O saturation. The gene abundance of nosZ II was significantly higher than that of nosZ I in the estuary, and the nosZ II/nosZ I abundance ratio was positively correlated with N₂O reduction potential. Furthermore, the community composition of NosZ-I- and NosZ-II-type N₂O-reducing bacteria shifted from upstream to downstream. NosZ-II-type N₂O-reducing bacteria, especially Myxococcales, Thiotrichales, and Gemmatimonadetes species, contributed to the high N₂O reduction potential in the downstream. Our results suggest that NosZ-II-type N₂O-reducing bacteria play a dominant role in determining the release potential of N₂O from sediments in the Pearl River Estuary. This study provides a new insight into the function of microbial N₂O reduction in estuarine ecosystems.

Keywords: N(2)O emission; N(2)O reduction; NosZ-I-Type N(2)O-reducing bacteria; NosZ–II–Type N(2)O-Reducing bacteria; Pearl river estuary.