Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 May;32(4):525-535.
doi: 10.1007/s10646-023-02655-6. Epub 2023 Apr 29.

Single and joint toxic effects of thyroid hormone, levothyroxine, and amiodarone on embryo-larval stages of zebrafish (Danio rerio)

Thamiris Pinheiro Santos  1   2 , Paulo Eduardo da Silva Bastos  1 , Jadson Freitas da Silva  1 , Stefânia Maria de Medeiros Vieira  1 , Marília Cordeiro Galvão da Silva  1 , André Lucas Corrêa de Andrade  1   2 , Renata Meireles Oliveira Padilha  1 , Amanda Rodrigues Dos Santos Magnabosco  1 , Marilia Ribeiro Sales Cadena  3 , Pabyton Gonçalves Cadena  4   5
Affiliations

Single and joint toxic effects of thyroid hormone, levothyroxine, and amiodarone on embryo-larval stages of zebrafish (Danio rerio)

Thamiris Pinheiro Santos et al. Ecotoxicology. 2023 May.

Abstract

This study evaluates single and joint endocrine disruptor toxicities of thyroid hormone, levothyroxine, and amiodarone in the embryo-larval stages of Danio rerio. Single toxicity experiments were carried out in concentrations based on the environmental concentration and increasing concentrations of 10, 100, and 1000 times the environmental concentration. Joint toxicity experiments evaluated the combined effects of these compounds. Toxic effects were examined during zebrafish embryonic development, and the parameters analyzed were apical sublethal, teratogenicity, mortality endpoints, and morphometry. Thyroid hormone exhibited the highest toxicity. However, the results showed that the environmental concentrations for all 3 compounds had low risk in relation to the parameters studied, such as teratogenic effects and morphometry. The larvae were more affected than embryos, where embryos needed higher concentrations in all experiments, possibly due to the absence of the chorion. The same type of effects were observed in the joint toxicity test, except that a possible antagonistic effect was detected. However, high concentrations showed stronger effects of these toxic compounds on fish development.

Keywords: Antagonistic effect; Aquatic animals; Developmental toxicity; Endocrine disruptors; Zebrafish.

PubMed Disclaimer

References

    1. Abdallah MAM (2016) Endocrine disruptors as pollutants in marine ecosystem: a case study in Egypt. Open Biotechnol J 10:131–150 - DOI
    1. Arbogast P, Flamant F, Godement P, Glösmann M, Peichl L (2016) Thyroid hormone signaling in the mouse retina. PLoS One 11:e0168003. https://doi.org/10.1371/journal.pone.0168003 - DOI
    1. Bartalena L, Bogazzi F, Chiovato L, Hubalewska-Dydejczyk A, Links TP, Vanderpump M (2018) European ThyroidAssociation (ETA) guidelines for the management of amiodarone-associated thyroid dysfunction Eur Thyroid J 7:55–66. https://doi.org/10.1159/000486957 - DOI
    1. Cadena PG, Cadena MRS, Sarmah S, Marrs JA(2020a) Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD). Reprod Toxicol 96:249–257. https://doi.org/10.1016/j.reprotox.2020.07.013 - DOI
    1. Cadena PG, Sales Cadena MR, Sarmah S, Marrs JA (2020b) Protective effects of quercetin, polydatin, and folic acid and their mixtures in a zebrafish (Danio rerio) fetal alcohol spectrum disorder model. Neurotoxicol Teratol 82:106928. https://doi.org/10.1016/j.ntt.2020.106928 - DOI

LinkOut - more resources