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. 2023 Apr 24:16:1079-1087.
doi: 10.2147/CCID.S405491. eCollection 2023.

The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

Jiamin Wen  1 , Jitian Weng  2 , Wei Lu  3 , Xiaohua Tao  3 , Hao Cheng #  4 , Yi Tang #  3
Affiliations

The Expression of Plasmacytoid Dendritic Cells and TLR7/9-MyD88-IRAKs Pathway in Chronic Eczema Lesions

Jiamin Wen et al. Clin Cosmet Investig Dermatol. .

Abstract

Purpose: To investigate the expression of Plasmacytoid Dendritic Cells (pDCs) and TLR7/9-MyD88-IRAKs pathway in chronic eczema lesions.

Patients and methods: Lesional tissues and the surrounding healthy tissues were collected from 25 individuals with chronic eczema, and immunohistochemistry was used to detect and comparatively analyze the expression profile of CD123, CD2AP, toll-like receptor 7 (TLR7), toll-like receptor 9 (TLR9) along with interleukin-1 receptor-associated kinase 1 (IRAK1) and interferon regulatory factor 7 (IRF7) in signaling pathways.

Results: The positive rates of CD2AP + pDC and CD123 + pDC in lesional tissues were significantly elevated compared to the surrounding healthy tissues (P < 0.05). They were distributed in both the epidermal and dermal layers of the lesional tissue, but the majority were in the dermal layer. The TLR7, TLR9, IRAK1 and IRF7 were more expressed in dermal layers of the lesional tissue with higher positive rates of expression compared to the surrounding healthy tissues (P < 0.05). IRAK1 and IRF7 were expressed in a small amount in the epidermal layer with higher positive rates of expression than in the surrounding healthy tissues (P < 0.05), while the positive rates of TLR7 and TLR9 expression in the epidermal layer were not statistically different from those in the surrounding healthy tissues (P > 0.05).

Conclusion: PDC and TLR7/9-MyD88-IRAKs pathways are actively expressed in chronic eczema lesions and may be involved in pathogenesis and disease progression.

Keywords: eczema; immunohistochemistry; plasmacytoid dendritic cells; toll-like receptor 7; toll-like receptor 9.

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Conflict of interest statement

Dr Yi Tang reports grants from the fund of Health and Family Planning Commission of Zhejiang Province (2019PY001), during the conduct of the study. The authors report no other conflicts of interest in this work.

Figures

Figure 1
Figure 1
Expressions of CD2AP in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) More CD2AP+ monocyte-like cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More CD2AP+ monocyte-like cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of CD2AP+ monocyte-like cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
Figure 2
Figure 2
Expressions of CD123 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few CD123+ monocyte-like cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More CD123+ monocyte-like cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of CD123+ monocyte-like cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
Figure 3
Figure 3
Expressions of TLR7 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few TLR7+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) A few TLR7+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Few TLR7+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
Figure 4
Figure 4
Expressions of TLR9 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) A few TLR9+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) A few TLR9+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Few TLR9+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
Figure 5
Figure 5
Expressions of IRAK1 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion. (c) Perilesional normal skin. (a) More IRAK1+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More IRAK1+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of IRAK1+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
Figure 6
Figure 6
Expressions of IRF7 in chronic eczema lesions and the perilesional normal skin at the edge (SP *400). (a) Epidermic of skin lesion. (b) Dermis of skin lesion (c) Perilesional normal skin. (a) More IRF7+ cells with brown cytoplasm were observed in the epidermal of chronic eczema lesions. (b) More IRF7+ cells with brown cytoplasm were observed in the dermal papillary layer of chronic eczema lesion. (c) Almost no of IRF7+ cells with brown cytoplasm were observed in both epidermal and papillary layer of perilesional normal skin of the lesion.
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