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. 2023 May 1:38:e381523.
doi: 10.1590/acb381523. eCollection 2023.

Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue

Veysel Toprak  1 , Senem Alkan Akalın  2 , Ece Öcal  3 , Yunus Çavuş  1 , Engin Deveci  4
Affiliations

Biochemical and immunohistochemical examination of the effects of ephedrine in rat ovary tissue

Veysel Toprak et al. Acta Cir Bras. .

Abstract

Purpose: It was aimed to investigate the biochemical and immunohistochemical effects of ephedrine (EPH) in bilateral ovariectomized rats.

Methods: Twenty-four Sprague Dawley female rats were divided into three groups: control group: The abdomen was opened and closed without any treatment; ischemia-reperfusion (IR) group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days.

Results: Biochemical parameters were statistically significant in group comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and antral follicle cells and inflammatory cells around blood vessels were seen in IR group. Negative IL-6 expression was observed in seminal epithelial cells, preantral and antral follicle cells in IR+EPH group. While caspase-3 activity increased in granulosa cells and stromal cells in IR group, caspase-3 expression was negative in preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH group.

Conclusions: The effect of apoptosis, which occurs with the signaling that starts in the cell nucleus, caused the cessation of the stimulating effect at the nuclear level after EPH administration, and a decrease in the antioxidative effect in IR damage and inflammation in the apoptotic process.

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Conflict of interest statement

Conflict of interest: No potential conflict of interest was reported by the authors.

Figures

Figure 1
Figure 1. IL-6 and Caspase-3 immunostaining in Control, IR and IR+EPH groups. (a) IL-6 expression in Control group; (b) IL-6 expression in IR group; (c) IL-6 expression in IR+EPH group; (d) Caspase-3 expression in Control group; (e) Caspase-3 expression in IR group; (f) Caspase-3 expression IR+EPH group (Bar: 100 μm, magnification: 10×).
See this image and copyright information in PMC

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