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. 2023 Jul;98(1):e13279.
doi: 10.1111/sji.13279. Epub 2023 May 14.

Luminex Crossmatch in kidney transplantation

Radouan Fadi Ameur  1   2 , Lilya Meriem Berkani  1   2 , Brahim Belaid  1   2 , Khadidja Habchi  3   4 , Messaoud Saidani  3   4 , Reda Djidjik  1   2
Affiliations
Free article

Luminex Crossmatch in kidney transplantation

Radouan Fadi Ameur et al. Scand J Immunol. 2023 Jul.
Free article

Abstract

The introduction of the Luminex Crossmatch assay (LumXm) which uses Luminex bead technology, consists of extracting the donor's Human Leukocyte Antigen (HLA) molecules from their lymphocytes, and binding them to fluorescent beads that are put in contact with recipient's serum. HLA donor-specific antibodies (DSA) are detected using a fluorescent conjugate. The goal of our study is to determine the benefits of using LumXm in a renal transplantation algorithm. We tested 78 recipients' sera using the LumXm, and the results were compared with the Luminex single antigen bead assay (SAB) for all sera, as well as the Flow Cytometry Crossmatch (FCXM) for 46 sera. We compared our results with those of SAB using 3 cutoffs, the first being the manufacturer's criteria where sensitivity and specificity were at 62.5% and 91.3% respectively for HLA class 1, and 88.5% and 50.0% respectively for HLA class 2. When using the third cutoff criteria (≥2 Adjusted values + MFI [Mean fluorescence intensity] >500 + Neg MFI < 500), the sensitivity increased to 69.0% for HLA class 1 and decreased to 84.0% for HLA class 2, while the specificity increased for HLA class 1 and 2. When comparing with FCXM, the 3 assays agreed in 55.8% of results for class 1 and 2 alike. However, major discrepancies were found for two groups in HLA class 1 and one in HLA class 2. The LumXm when used with other techniques to overcome its' weak points, can provide an interesting insight into the patient's HLA-DSA profile.

Keywords: Luminex Crossmatch; anti-HLA antibodies; crossmatch; donor-specific antibodies; kidney transplantation.

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References

REFERENCES

    1. Patel R, Terasaki PI. Significance of the positive crossmatch test in kidney transplantation. N Engl J Med. 1969;280:735-739.
    1. Terasaki PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature. 1964;204(4962):998-1000.
    1. Fuller TC, Cosimi AB, Russell PS. Use of an antiglobulin-ATG reagent for detection of low levels of alloantibody. Improvement of allograft survival in presensitized recipients. Transplant Proc. 1978;10(2):463-466.
    1. Kissmeyer-Nielsen F, Olsen S, Petersen VP, Fjeldborg O. Hyperacute rejection of kidney allografts associated with pre-existing humoral antibodies against donor cells. Lancet. 1966;1:662-665.
    1. Williams GM, Hume DM, Hudson RP Jr, Morris PJ, Kano K, Milgorm F. Hyperacute renal-homograft rejection in man. N Engl J Med. 1968;279:611-618.